Human Alu DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® Stain and 0.5-mL Tubes (with voucher)

Advanced—For experienced high school and college classes; requires some technical skill.

• Simple, robust protocol is ideal for classroom use
• Incorporates Hardy-Weinberg equilibrium and chi-square analysis
• Helps demonstrate real-world applications of PCR
• Discover how Alu insertions are used to track world population migrations
• CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles, and each student determines his/her own genotype. The experiment provides an introduction to human population genetics as participants learn to score genotypes and calculate genotype and allele frequencies.

Kit contains instructions, a CD-ROM, and materials for 25 students to perform DNA extraction, amplification, and electrophoresis (staining with CarolinaBLU®). Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).