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AdvancedFor experienced high school and college classes; requires some technical skill.
Students use a classroom-friendly protocol involving saline mouthwash or a hair sample and Chelex® extraction to obtain a sample of their own DNA. This high-quality kit then has students amplify and sequence a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome. Kit includes enough materials for 25 students to perform the DNA extraction and amplification process.
AdvancedFor experienced high school and college classes; requires some technical skill.
Generate excitement by having your students amplify their own mitochondrial DNA for sequencing!
Kit contains instructions and materials for 25 students to perform the DNA extraction and amplification step of the protocol. Kit does not include materials for electrophoresis. Note: Kit is shipped with perishable materials.
Components | Qty | Included? |
---|---|---|
mtDNA Primer/Loading Dye Mix, 700 µL ![]() |
1 | Included |
pBr322/BstNI Markers, 0.075 µg/µL, 130 μL ![]() |
1 | Included |
Proteinase K, 100 µg/mL, 5 mL ![]() |
1 | Included |
Chelex® 100 Resin, 1.5 g | 1 | Included |
PCR Tubes Containing Ready-to-Go™ Beads (0.2-mL capacity) | 25 | Included |
Mineral Oil, 5 mL | 1 | Included |
Teacher's Manual with Reproducible Student Guide | 1 | Included |
Aluminum Foil | Needed, Not Included | |
Beakers Containing Ice | Needed, Not Included | |
Programmable DNA Thermal Cycler | Needed, Not Included | |
Electrophoresis Chambers | Needed, Not Included | |
Electrophoresis Power Supplies | Needed, Not Included | |
Forceps | Needed, Not Included | |
Laboratory Markers (1 per student) | Needed, Not Included | |
Micropipets and Tips, 1001,000 µL | Needed, Not Included | |
Micropipets and Tips, 110 µL or 120 µL | Needed, Not Included | |
Microcentrifuge Tube Racks | Needed, Not Included | |
Microcentrifuge Tubes, 1.5 mL | Needed, Not Included | |
Paper Cups | Needed, Not Included | |
Razor Blade or Scalpel | Needed, Not Included | |
Saline Solution, 0.9% | Needed, Not Included | |
Boiling Water Baths | Needed, Not Included | |
Water Baths, 37° and 65° C | Needed, Not Included | |
UV Transilluminator (for ethidium bromide stain) or White Light Box (for CarolinaBLU™ stain) | Needed, Not Included | |
Vortexer (optional) | Needed, Not Included | |
Centrifuge for 15-mL Tubes (minimum 500 x g; optional but useful) | Needed, Not Included | |
Centrifuge for 1.5-mL Tubes (optional but useful) | Needed, Not Included | |
Camera (optional) | Needed, Not Included |
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.