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Exploring the CRISPR-Cas Defense System Kit (with perishables)

Item # 211772P Exclusive This product is exclusive to Carolina Biological Supply.

Intermediate - Easy to perform; requires some basic training in microbiology.
Students conduct experiments and analyze data over the course of this 5-day lab to understand the relationship between a phage called Andhra and its staphylococcus host. Their goal is to answer the driving question, How can we use evidence to model phage-host interactions and therefore explain the impact of a CRISPR-Cas system on bacterial survival?

Price

$119.00

In stock and available to ship.

Intermediate - Easy to perform; requires some basic training in microbiology.
Bring new gene-editing technology into your classroom!
Your students walk in the footsteps of the scientists that first discovered this exciting gene-editing technology. Originally discovered as an integral part of the immune system of bacteria, you and your class explore the CRISPR-Cas system and its role in defending the host bacteria from viral bacteriophages. Bacteriophages outnumber bacteria 10:1 and have evolved an intricate immune system to combat invading phages. One such system, the CRISPR-Cas, uses small pieces of RNA to detect phages with matching DNA sequences, and destroys them.

Over the course of this 5-day lab, students conduct experiments and analyze data to understand the relationship between a phage called Andhra and its staphylococcus host and answer the driving question, How can we use evidence to model phage-host interactions and therefore explain the impact of a CRISPR-Cas system on bacterial survival? Note: Kit includes perishable materials. Please order 2 weeks prior to the requested delivery date. Perishables are only shipped on Mondays, Tuesdays, and Wednesdays. Plan to use the perishable materials within 1 week of receiving them. S. epidermidis is classified as nonpathogenic and can be shipped to schools in accordance with guidelines of the US Department of Health and Human Services. However, the strains used in this kit are biofilm forming bacteria that have been identified as resistant to several antibiotics. Use of an autoclave to dispose of microbiological waste is recommended. Refer to the kit teacher's manual for safety precautions, handling, and other safety protocols.

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.

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This item ships with living or perishable materials (all   items). Please select a delivery date during Checkout.

Components Qty Included?
S. epidermidis LM1680 Culture  1 Included
S. epidermidis Strain A Culture  1 Included
S. epidermidis Strain B Culture  1 Included
Phage Andhra Lysate, 1.5 mL (1 x 104 pfu/mL) (pfu=plaque-forming unit)   1 Included
Powdered Chloramphenicol, 100 mg  1 Included
Calcium Chloride, 2 mL 1 Included
Heart Infusion Agar, 250 mL 1 Included
Tryptic Soy Agar, 300 mL 5 Included
Tryptic Soy Broth, 125 mL 1 Included
Sterile Petri Dishes, 100 mm 60 Included
Sterile Culture Tubes, 15 mL 75 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Sterile Microcentrifuge Tubes Needed, Not Included
Sterile Serological Pipets Needed, Not Included
Sterile Micropipet Tips Needed, Not Included
Pipetting Device Needed, Not Included
Micropipets, 2–20 µL Needed, Not Included
Micropipets, 10–200 µL Needed, Not Included
Micropipets, 100–1000 µL Needed, Not Included
Beakers, 600 mL 8 Needed, Not Included
37° C Microbiological Plate Incubator 1 Needed, Not Included
37° C Shaking Incubator (optional) 1 Needed, Not Included
Refrigerator 1 Needed, Not Included
Boiling Water Bath (or 4 hot plates) Needed, Not Included
Autoclave 1 Needed, Not Included
Disposable Gloves Needed, Not Included
Lab Coats Needed, Not Included
Safety Glasses Needed, Not Included
Test Tube Racks 8 Needed, Not Included
Microcentrifuge Tube Racks 4 Needed, Not Included
Heat-Resistant Mitt Needed, Not Included
70% Ethanol, 10% Household Bleach Solution, or Lysol® Disinfectant Needed, Not Included
95% Ethanol Needed, Not Included
Paper Towels Needed, Not Included
Permanent Markers 8 Needed, Not Included
Large Plastic Bin (dedicated for bacterial plate transport/storage) 1 Needed, Not Included
Waste Containers (for biological trash) 8 Needed, Not Included
Clear Autoclavable Bag(s) Needed, Not Included
Bunsen Burner 1 Needed, Not Included