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Exploring the CRISPR-Cas Defense System Kit (with voucher)

(in stock)


Intermediate - Easy to perform; requires some basic training in microbiology.
Bring new gene-editing technology into your classroom!
Your students walk in the footsteps of the scientists that first discovered this exciting gene-editing technology. Originally discovered as an integral part of the immune system of bacteria, you and your class explore the CRISPR-Cas system and its role in defending the host bacteria from viral bacteriophages. Bacteriophages outnumber bacteria 10:1 and have evolved an intricate immune system to combat invading phages. One such system, the CRISPR-Cas, uses small pieces of RNA to detect phages with matching DNA sequences, and destroys them.

Over the course of this 5-day lab, students conduct experiments and analyze data to understand the relationship between a phage called Andhra and its staphylococcus host and answer the driving question, How can we use evidence to model phage-host interactions and therefore explain the impact of a CRISPR-Cas system on bacterial survival? Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishables. S. epidermidis is classified as nonpathogenic and can be shipped to schools in accordance with guidelines of the US Department of Health and Human Services. However, the strains used in this kit are biofilm forming bacteria that have been identified as resistant to several antibiotics. Use of an autoclave to dispose of microbiological waste is recommended. Refer to the kit teacher's manual for safety precautions, handling, and other safety protocols.


What’s Included:
  • S. epidermidis LM1680 Culture
  • S. epidermidis Strain A Culture
  • S. epidermidis Strain B Culture
  • 1 Phage Andhra Lysate, 1.5 mL (1 x 104 pfu/mL) (pfu=plaque-forming unit)
  • 1 Powdered Chloramphenicol, 100 mg
  • 1 Calcium Chloride, 2 mL
  • 1 Heart Infusion Agar, 250 mL
  • 5 Tryptic Soy Agar, 300 mL
  • 1 Tryptic Soy Broth, 125 mL
  • 60 Sterile Petri Dishes, 100 mm
  • 75 Sterile Culture Tubes, 15 mL
  • 1 Carolina Science Online® Resources (1-year subscription)
  • 1 Teacher’s Manual with Reproducible Student Guide
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Needed But Not Included:
  •  Sterile Microcentrifuge Tubes
  •  Sterile Serological Pipets
  •  Sterile Micropipet Tips
  •  Pipetting Device
  •  Micropipets, 2–20 µL
  •  Micropipets, 10–200 µL
  •  Micropipets, 100–1000 µL
  • 8 Beakers, 600 mL
  • 1 37° C Microbiological Plate Incubator
  • 1 37° C Shaking Incubator (optional)
  • 1 Refrigerator
  •  Boiling Water Bath (or 4 hot plates)
  • 1 Autoclave
  •  Disposable Gloves
  •  Lab Coats
  •  Safety Glasses
  • 8 Test Tube Racks
  • 4 Microcentrifuge Tube Racks
  •  Heat-Resistant Mitt
  •  70% Ethanol, 10% Household Bleach Solution, or Lysol® Disinfectant
  •  95% Ethanol
  •  Paper Towels
  • 8 Permanent Markers
  • 1 Large Plastic Bin (dedicated for bacterial plate transport/storage)
  • 8 Waste Containers (for biological trash)
  •  Clear Autoclavable Bag(s)
  • 1 Bunsen Burner
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Return Policy:

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