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Students investigate the relationship among a recessive gene (Adh−), its enzyme product (alcohol dehydrogenase, ADH), and the resulting phenotype. ADH-negative mutants cannot break down the ethanol they consume, leading to intoxication and, ultimately, death from alcohol poisoning. Students make observations over a 24-hr period, requiring them to take flies home overnight. For 8 groups of up to 4 students each; requires 2–3 class periods to complete. Flies must be subcultured before beginning the activity. Kit includes: Adh- and Adh+ (wild-type) Drosophila cultures, FlyNap(r) kit, 48 culture vials and vial plugs, instant Drosophila medium, graduated pipets, Drosophila sorting brushes, and Carolina(tm) Drosophila manual. Needed but not included:100 cotton balls and 160 mL ethanol.
This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).
Components | Qty | Included? |
---|---|---|
Adh- and wild-type (Adh+) Drosophila Cultures | Included | |
FlyNap® Kit | Included | |
Culture Vials | 48 | Included |
Vial Plugs | 48 | Included |
Instant Drosophila Medium | Included | |
Graduated Pipets | 8 | Included |
Drosophila Sorting Brushes | 8 | Included |
Carolina™ Drosophila Manual | Included | |
Cotton Balls | 100 | Needed, Not included |
160 mL Ethanol | Needed, Not included |