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Evolution in Real Time: Bacteria and Antibiotic Resistance 8-Station Kit (with prepaid coupon)

Item # 171206 Exclusive This product is exclusive to Carolina Biological Supply. With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.

Product Highlights

  • Investigate the evolution of a bacterial population's resistance to antibiotics over generations.
  • Simulate the process of natural selection using a robust protocol designed for the classroom.
  • High school laboratory investigation with enough materials for 8 lab groups.
  • Includes a prepaid coupon to request perishable materials later at your convenience.
  • Carolina Kits 3D®—Labs that use phenomena to support NGSS and 3-dimensional instruction.



In stock and available to ship.

This lab serves as a demonstration of natural selection and of evolution occurring over a short span of time. Students examine the evolution of bacterial populations grown both in the absence of, and presence of, an antibiotic.

Time Requirement
Total, 270 minutes over 2 weeks. Setting up the experiment, 1 lab period. Collecting data over the next 5 days, 15 to 20 minutes a lab period. Discussion and assessment, 2 to 3 lab periods.

Digital Resources
Includes 1-year access to digital resources that support 3-dimensional instruction for NGSS. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, phenomenon videos, simulations, and post-lab analysis and assessments.

Performance Expectation(s)
HS-LS4-2 and HS-LS4-3

Crosscutting Concepts
Cause and Effect

Disciplinary Core Ideas
LS4.B: Natural Selection
LS4.C: Adaptation

Science and Engineering Practices
Engaging in Argument from Evidence

Learning Objectives

  • Measure the differences in 2 populations that develop in response to different selective pressures.
  • Explain the process of natural selection and how it affects population genetics.
  • Describe bacterial antibiotic resistance and how and why it is maintained.
  • Use sterile technique, perform a serial dilution, and plate bacteria.

Prerequisite Knowledge and Skills
Students need to be familiar with bacteria and have a basic knowledge of their structure and genetics. They should have a working knowledge of genes, mutation, and proteins (including enzymes). They also need a preliminary knowledge of natural selection and evolution. Students should be able to use a micropipet and have some knowledge of sterile technique.

  • This item is only available from Carolina Biological Supply Company.

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
MM294 Slant Culture 1 Included
MM294/pAMP Slant Culture 1 Included
Ampicillin Vial, 10 mg/mL, 4 mL 1 Included
Ampicillin Vial, 100 mg/mL, 200 µL 8 Included
Glass Beads, Pack 3 Included
Culture Tubes 160 Included
Sterile Petri Dishes 40 Included
Sterile LB Agar, Bottle, 400 mL 2 Included
Sterile LB Broth, 50 mL 8 Included
Disposable Inoculating Loops 20 Included
Nichrome Wire Inoculating Loop 1 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Incubator, 37° C Needed, Not Included
Pipets or Measuring Device, 1 and 2 mL Needed, Not Included
Micropipets for 10-, 20-, 22-, and 100-µL Volumes Needed, Not Included
Micropipet Tips Needed, Not Included
Microwave or Boiling-Water Bath Needed, Not Included
Bunsen Burners Needed, Not Included
Waste Containers Needed, Not Included
Household Bleach or Ethanol Needed, Not Included
Permanent Markers Needed, Not Included
Culture Tube Racks Needed, Not Included
Hot Pad or Gloves Needed, Not Included
Microcentrifuge Tubes, Sterile Needed, Not Included