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Green Gene Colony Transformation 8-Station Kit, Module 1 (with perishables)

$122.55
Product Highlights
  • Teach transformation, protein purification, and protein gel electrophoresis
  • Module 1 of 3 sequential modules, allowing a customizable learning experience
  • Refill of perishables is available separately
(in stock)

Description

Product Details
Intermediate—Easy to perform; requires some background knowledge.

Bring genetic engineering into your classroom with the Green Gene Colony Transformation Kit. Developed with the DNA learning center, this kit allows students to induce bacteria to accept and express the gene for green fluorescent protein (GFP) from sea jellies causing the genetically modified bacteria they produce to visibly glow green. Kit includes perishable materials.

This kit is Module 1 of a 3-module sequence and provides the materials necessary for performing transformation. To perform protein purification after completing this experiment, obtain Module 2 (item #211072P or #211072), and to run electrophoresis on the purified protein, obtain Module 3 (item #211073P or #211073).

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

Product Features

  • Green color is visible in normal light—no UV lighting needed
  • Pre-aliquoted and prepared reagents minimize teacher prep time
  • Designed to match the traditional AP® Biology Lab 6
  • Colonies will fluoresce under UV light
  • Designed for 8 student groups/lab stations
  • Plasmid contains gene for ampicillin resistance

Time Requirement
Teacher prep, prepare LB agar plates and streak start plates. Performing lab, approximately one to two 60-minute class periods. Post lab results, approximately one 60-minute class period.

Digital Resources
Includes 1-year access to digital resources that include a teacher’s manual, student guide, fill-in answer sheets, whiteboard resources, videos, interactive digital prelab activity, and more.

Performance Expectation(s)
HS-LS1-1
HS-LS3-1

Crosscutting Concepts
Cause and Effect

Disciplinary Core Ideas
HS LS1.A: Structure and Function

Science and Engineering Practices
Constructing Explanations and Designing Solutions

Learning Objectives

  • Explain how one organism can be genetically engineered to produce a protein from a different organism.
  • Draw connections between the techniques performed in this laboratory and the development of current critical pharmaceuticals.
  • Explain the use and importance of selective marker genes in a bacterial transformation.

Prerequisite Knowledge and Skills
Students should have a basic knowledge of cell structure and function, cell wall and membrane structure and function, and a working knowledge of protein synthesis.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Culture 
  • 1 pGREEN Plasmid Solution, 200 µL 
  • 1 Ampicillin, 4 mL 
  • 2 Sterile LB Agar, 400 mL
  • 40 Sterile Petri Dishes
  • 8 50 mM Calcium Chloride, 3 mL
  • 8 LB Broth, 3 mL
  • 16 Transformation Tubes, 15 mL
  • 48 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 28 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
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Needed But Not Included:
  • 1 Boiling Water Bath or Microwave (for melting agar)
  • 8 Beakers, 600 mL
  • 1 Container (for glass beads)
  • 8 Culture Tube Racks
  • 8 Wax Markers
  • 1 Masking Tape
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Parafilm® or Plastic Wrap
  •  Bleach Solution, 10%
  •  Incubator (optional)
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Return Policy:

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