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Green Gene Colony Transformation 8-Station Kit, Module 1 (with prepaid coupon)

Item # 211082 Exclusive This product is exclusive to Carolina Biological Supply. With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.

Designed to match traditional AP® Biology Lab 6. This kit clearly demonstrates the induced uptake of a jellyfish gene by E. coli. The 8-Station Kit includes enough materials for 8 teams of students. Includes prepaid coupon to request perishable materials later at your convenience.



In stock and available to ship.

An eye-catching example of the genetic basis of inheritance

  • Green color is clearly visible in normal classroom light
  • No UV lighting needed
  • Pre-aliquoted and prepared reagents minimize teacher prep time
Designed to match traditional AP® Biology Lab 6. Open your students' eyes to the genetic basis of inheritance with this simple lab. Developed in cooperation with the DNA Learning Center, this kit clearly demonstrates the induced uptake of a jellyfish gene by E. coli. Students observe the phenotypic effect of adding the gene for GFP (green fluorescent protein). GFP is expressed on plain LB—no color development substrate or induction reagent is needed. Your students can easily view the "green" effects in normal classroom lighting. Colonies will fluoresce under UV light. The plasmid containing the GFP gene also contains an ampicillin resistance gene, so ampicillin can be used to select for transformants. Lab procedure is easy to follow. The 8-Station Kit includes enough materials for 8 teams of students. Includes prepaid coupon to request perishable materials later at your convenience.

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

  • This item is only available from Carolina Biological Supply Company.

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
E. coli Culture 1 Included
pGREEN Plasmid Solution, 200 µL 1 Included
Ampicillin, 4 mL 1 Included
Sterile LB Agar, 400 mL 2 Included
Sterile Petri Dishes 40 Included
50 mM Calcium Chloride, 3 mL 8 Included
LB Broth, 3 mL 8 Included
Transformation Tubes, 15 mL 16 Included
Sterile Transfer Pipets, 1 mL 48 Included
Glass Beads, 1 oz 1 Included
Sterile Transfer Loops 28 Included
Wire Inoculating Loop 1 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Boiling Water Bath or Microwave (for melting agar) 1 Needed, Not included
Beakers, 600 mL 8 Needed, Not included
Container (for glass beads) 1 Needed, Not included
Culture Tube Racks 8 Needed, Not included
Wax Markers 8 Needed, Not included
Masking Tape 1 Needed, Not included
Bunsen Burner 1 Needed, Not included
Water Bath, 42°C 1 Needed, Not included
Autoclavable Disposable Bag 1 Needed, Not included
Ice Needed, Not included
Parafilm® or Plastic Wrap Needed, Not included
Bleach Solution, 10% Needed, Not included
Incubator (optional) Needed, Not included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.

About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.