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Glow-in-the-Dark Transformation 4-Station Student Kit (with perishables)

$85.00

Description

Intermediate - Easy to perform; requires some background knowledge.

Bacteria that produce light are very common in the ocean. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant. Kit includes perishable materials, but the kit and the perishable components will ship separately and may arrive on different days. Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.

Specifications

Shipping Information or Purchase Restrictions
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Culture  (perishable item)
  • 1 pVIB Plasmid Solution, 200 µL  (perishable item)
  • 1 Ampicillin, 4 mL  (perishable item)
  • 1 Sterile LB Agar, 400 mL
  • 20 Sterile Petri Dishes
  • 4 50 mM Calcium Chloride, 3 mL
  • 4 LB Broth, 3 mL
  • 8 Transformation Tubes, 15 mL
  • 24 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 15 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
Needed But Not Included:
  • 1 Boiling Water Bath or Microwave (for melting agar)
  • 4 Beakers, 600 mL
  • 1 Container (for glass beads)
  • 4 Culture Tube Racks
  • 4 Wax Markers
  • 1 Masking Tape
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Parafilm® or Plastic Wrap
  •  Bleach Solution, 10%
  •  Incubator (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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