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800.334.5551

Purification of Green Fluorescent Protein 8-Station Kit, Module 2 (purification) (with voucher)

$63.35
(in stock)

Description

Intermediate - Requires some background knowledge.

This kit module is great for teaching:

  • The molecular basis of heredity
  • The relationship between genes and proteins
  • How E. coli is used to engineer proteins useful in medicine and manufacturing
  • Three techniques - transformation, protein purification, and protein gel electrophoresis - commonly used in biological research and its areas of application

This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 2 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P).

In this activity students take their successfully transformed bacteria and use HIC resin to conduct a batch purification technique. This purification process doesn't require the time or expense typically associated with column-based purification protocols and instead produces quick results in 1 class period (40 minutes).

Note: Kit includes a voucher to request perishable material later at your convenience. Contact us or return the voucher to request delivery of perishable materials.

Specifications

What’s Included:
  • 1 Ampicillin, 4 mL (10mg/mL)
  • 8 HIC Bead Resin, 300 µL
  • 1 Equilibration Buffer, 10 mL
  • 8 Lysis Buffer, 600 µL
  • 8 Binding Buffer, 400 µL
  • 8 Wash Buffer, 600 µL
  • 8 TE Buffer, 600 µL
  • 1 Luria Broth (LB), 20 mL
  • 8 Culture Tubes
  • 48 Microcentrifuge Tubes, 1.5 mL
  • 1 Teacher’s Manual with Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  • 1 +pGREEN GFP Plate (from Module 1)
  • 1 Shaking Water Bath or Incubator
  •  Pipets, 100-1,000 µL
  •  Pipet Tips
  • 1 Microcentrifuge
  •  Permanent Markers
  •  Ice Buckets
  •  UV Light Source
  •  Waste Containers
  •  Vortex (optional)
  • expand to see full list
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