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  • Purification of Green Fluorescent Protein 8-Station Kit, Module 3 (electrophoresis) (with prepaid coupon)

Purification of Green Fluorescent Protein 8-Station Kit, Module 3 (electrophoresis) (with prepaid coupon)

Item # 211073 Exclusive This product is exclusive to Carolina Biological Supply.

Bringing genetic engineering and protein purification into your classroom has never been simpler. Module 3 is designed to allow students to conduct electrophoretic analysis of their purified GFP protein to confirm successful isolation. This lab activity requires prior completion of Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P), and Module 2 (purification, item #211072 or #211072P).
Price

$117.30

In stock and available to ship.
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Overview

Advanced - For more experienced classes; requires some technical skill.

This kit module is great for teaching:

  • The molecular basis of heredity
  • The relationship between genes and proteins
  • How E. coli is used to engineer proteins useful in medicine and manufacturing
  • Three techniques - transformation, protein purification, and protein gel electrophoresis - commonly used in biological research and its areas of application

This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 3 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P), and Module 2 (purification, item #211072 or #211072P).

In this activity students take their successfully purified GFP and run a polyacrylamide gel to determine the results of their purification technique. Students analyze the band of purified GFP, approximately 27kDa, and compare it to the banding pattern of unpurified cell lysate to determine the effectiveness of their purification process. This activity typically takes 90 minutes for students to prepare, load, and run their gels followed by a COOMASSIE® blue staining process.

Note: Kit includes a prepaid coupon to request perishable material later at your convenience. Contact us or return the coupon to request delivery of perishable materials. Refrigerate polyacrylamide gels and protein marker until use.
  • This item is only available from Carolina Biological Supply Company.
  • Limited Qty: Flammable Liquid - This product is/contains a limited quantity of a DOT regulated hazardous material. This item will not incur a hazmat fee when shipped by ground. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.

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What's Included

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Polyacrylamide Gels 3 Included
Protein Marker, 30 µL 1 Included
Tris-Glycine-SDS Buffer, 500 mL 1 Included
4x Loading Dye, 100 µL 4 Included
COOMASSIE® Blue Protein Staining Solution, 500 mL 1 Included
Microcentrifuge Tubes, 1.5 mL 24 Included
Staining Trays 3 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Purified GFP and Cell Lysate (from Module 2) Needed, Not Included
Hot Plate or Heating Block, 95° C Needed, Not Included
Permanent Markers Needed, Not Included
Pipets, 2-20 µL Needed, Not Included
Pipet Tips Needed, Not Included
Protein Gel Electrophoresis Apparatus and Power Supply Needed, Not Included
Distilled or Deionized Water Needed, Not Included
Gloves Needed, Not Included
Ice Buckets Needed, Not Included
White Light Transilluminator (optional) Needed, Not Included
Digital Camera (optional) Needed, Not Included

Ratings & Reviews

DNA Learning Center

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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