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pBLU® Colony Transformation 4-Station Kit (with voucher)

$97.20
(in stock)

Description

Thanks to the dramatic color change and ampicillin, students clearly see the results of introducing new genes into live bacteria

  • Great for teaching the molecular basis of inheritance
  • Simple, straightforward procedure
  • Minimizes teacher prep time
Designed to match traditional AP® Biology Lab 6. Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. Transformants appear as white colonies on the ampicillin medium and as dramatically blue colonies on the ampicillin/X-gal medium. The 4-Station Kit includes enough materials for 4 teams of students. Includes voucher to request perishable materials later at your convenience.

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

Specifications

What’s Included:
  • E. coli Culture
  • 1 pBLU® Plasmid Solution, 200 µL
  • 1 Ampicillin, 4 mL
  • 1 Ampicillin/X-gal Solution, 2% in DMF, 5.4 mL
  • 2 Sterile LB Agar, 400 mL
  • 25 Sterile Petri Dishes
  • 4 50 mM Calcium Chloride, 3 mL
  • 4 LB Broth, 3 mL
  • 8 Transformation Tubes, 15 mL
  • 24 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 15 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
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Needed But Not Included:
  • 1 Boiling Water Bath or Microwave (for melting agar)
  • 4 Beakers, 600 mL
  • 1 Container (for glass beads)
  • 4 Culture Tube Racks
  • 4 Wax Markers
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Parafilm® or Plastic Wrap
  •  Bleach Solution, 10%
  •  Incubator (optional)
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