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pClone: Exploring Promoters with Synthetic Biology

$6.15 - $243.75

Give your students the opportunity to learn and explore transcription regulation right in your classroom. This unique approach to synthetic biology was developed by college professors focused on creating a unique activity to demonstrate gene regulation. This multi-part lab will expose students to cloning, restriction enzymes, transformation, microbiology, and so much more in an effective classroom protocol.

Description

Advanced—For experienced high school and college classes; requires some technical skill.

  • Unique introduction to synthetic biology
  • Hands-on exploration of the role of promoters in gene regulation
  • Seamlessly blends together multiple areas of biology
  • Takes a traditional transformation lab to the next level
  • Lesson can be facilitated in 6 lab sessions, at most
  • Bring the unique area of synthetic biology into your classroom with this one-of-a-kind kit activity. Your students get the opportunity to take on the role of genetic engineers as they clone promoters into 2 different plasmids. This hands-on approach to transcription regulation exposes your class to a wide array of biological disciplines including biotechnology, molecular biology, and evolutionary biology.

    Students clone the promoters into the plasmids, pClone Red and pClone Blue, using a common cloning technique, Golden Gate Assembly (GGA). After cloning the promoters, students are tasked with performing a transformation on E. coli cells to produce new ampicillin-resistant colonies expressing their designed plasmids. Based on the orientation of the promoter, students then observe colonies that appear green (produce GFP), red (produce RFP), or blue (produce AmilCP blue). This gives them 3 distinct colonies to further assess using ImageJ software, which is freely available, to determine the level of protein expression.

    The kit includes enough materials for 8 groups of 2 to 4 students. The activities typically take 3 to 6 class periods for completion. Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact us or return the voucher to request delivery of the perishable materials.

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