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Give your students the opportunity to learn and explore transcription regulation right in your classroom. This unique approach to synthetic biology was developed by college professors focused on creating a unique activity to demonstrate gene regulation. This multi-part lab will expose students to cloning, restriction enzymes, transformation, microbiology, and so much more in an effective classroom protocol.
Advanced—For experienced high school and college classes; requires some technical skill.
Students clone the promoters into the plasmids, pClone Red and pClone Blue, using a common cloning technique, Golden Gate Assembly (GGA). After cloning the promoters, students are tasked with performing a transformation on E. coli cells to produce new ampicillin-resistant colonies expressing their designed plasmids. Based on the orientation of the promoter, students then observe colonies that appear green (produce GFP), red (produce RFP), or blue (produce AmilCP blue). This gives them 3 distinct colonies to further assess using ImageJ software, which is freely available, to determine the level of protein expression.
The kit includes enough materials for 8 groups of 2 to 4 students. The activities typically take 3 to 6 class periods for completion. Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact us or return the voucher to request delivery of the perishable materials.
Item #211150EBK
$6.15
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