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Purification of Green Fluorescent Protein 8-Station Kit, Module 3 (electrophoresis) (with perishables)

$120.80
(in stock)

Description

Advanced - For more experienced classes; requires some technical skill.

This kit module is great for teaching:

  • The molecular basis of heredity
  • The relationship between genes and proteins
  • How E. coli is used to engineer proteins useful in medicine and manufacturing
  • Three techniques - transformation, protein purification, and protein gel electrophoresis - commonly used in biological research and its areas of application

This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 3 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P), and Module 2 (purification, item #211072 or #211072P).

In this activity students take their successfully purified GFP and run a polyacrylamide gel to determine the results of their purification technique. Students analyze the band of purified GFP, approximately 27kDa, and compare it to the banding pattern of unpurified cell lysate to determine the effectiveness of their purification process. This activity typically takes 90 minutes for students to prepare, load, and run their gels followed by a COOMASSIE® blue staining process.

Note: Kit ships with the perishable materials included. Refrigerate polyacrylamide gels and protein marker until use.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon Limited Qty: Flammable Liquid - This product is/contains a limited quantity of a DOT regulated hazardous material. This item will not incur a hazmat fee when shipped by ground. Orders shipping to HI and AK are subject to review and may incur additional shipping charges. This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 3 Polyacrylamide Gels 
  • 1 Protein Marker, 30 µL 
  • 1 Tris-Glycine-SDS Buffer, 500 mL
  • 4 4x Loading Dye, 100 µL
  • 1 COOMASSIE® Blue Protein Staining Solution, 500 mL
  • 24 Microcentrifuge Tubes, 1.5 mL
  • 3 Staining Trays
  • 1 Teacher’s Manual with Reproducible Student Guide
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Needed But Not Included:
  •  Purified GFP and Cell Lysate (from Module 2)
  •  Hot Plate or Heating Block, 95° C
  •  Permanent Markers
  •  Pipets, 2-20 µL
  •  Pipet Tips
  •  Protein Gel Electrophoresis Apparatus and Power Supply
  •  Distilled or Deionized Water
  •  Gloves
  •  Ice Buckets
  •  White Light Transilluminator (optional)
  •  Digital Camera (optional)
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Return Policy:

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