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Transduction of an Antibiotic-Resistance Gene Kit (with voucher)

(in stock)


Intermediate—Easy to perform; requires some background knowledge.

In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage. For more advanced students, a discussion of nonsense mutations and nonsense suppressing mutations is included with the teacher background information. Kit includes a voucher to request perishable materials later at your convenience. Contact us or return voucher to request delivery of perishable materials. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).


What’s Included:
  • E. coli BE Slant Culture (voucher item)
  • E. coli CR63 Slant Culture (voucher item)
  • 1 Transducing Lysate, 300 µL (voucher item)
  • 2 Ampicillin, 10 mg/mL, 4 mL (voucher item)
  • 2 Glass Beads, 1 oz
  • 4 Luria Broth Agar, 400 mL
  • 3 Luria Broth, 50 mL
  • 4 Sterile Pipets, 1 mL
  • 2 Sterile Pipets, 10 mL
  • 100 Sterile Culture Tubes
  • 4 Inoculating Loops
  • 65 Sterile Petri Plates
  • 24 Sterile Transfer Pipets
  • 1 Digital Resource Instruction Card
  • 1 Teacher’s Manual and Reproducible Student Guide
Needed But Not Included:
  •  Micropipets (10 µL and 50 µL)
  •  Sterile Tips for Micropipets
  •  Sterile Microcentrifuge Tubes
  •  Pipet Bulb or Automatic Pipet
  • 8 Large Waste Beakers
  • 8 Large Glass Bead Collection Beakers
  • 1 Boiling Water Bath (or microwave)
  •  Test Tube Racks
  •  Permanent Marker
  •  Bunsen Burner
  •  Incubator, 37° C (optional)
  •  Water Bath, 55° C (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.


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