Identifying the Mutation in Non-Purple Stem Wisconsin Fast Plants® Extraction, Amplification, and Electrophoresis Kit with GelGreen™ Stain and 0.2-mL Tubes (with prepaid coupon) | Carolina.com

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Identifying the Mutation in Non-Purple Stem Wisconsin Fast Plants® Extraction, Amplification, and Electrophoresis Kit with GelGreen™ Stain and 0.2-mL Tubes (with prepaid coupon)

Item # 211465 New Online Only
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Advanced—For experienced high school and college classes; requires some technical skill.
Explore the connection between Mendelian genetics and molecular biology through the analysis of the mutation responsible for the non-purple stem trait in Fast Plants®. Students predict phenotype and genotype for stem color in the F2 generation of a monohybrid cross and propose an explanation as to why the anl allele leads to a non-purple stem phenotype, whereas the ANL allele leads to the purple stem phenotype.

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

Making a genotype-to-phenotype connection using Wisconsin Fast Plants®

This lab explores the connection between Mendelian genetics and molecular biology through the analysis of the mutation responsible for the non-purple stem trait in Wisconsin Fast Plants®. Students predict the phenotype and genotype for stem color in the F2 generation of a monohybrid cross and propose an explanation as to why the anl allele leads to a non-purple stem phenotype, whereas the ANL allele leads to the purple stem phenotype. Students finally plant F2 seeds on a monohybrid cross that will be used for phenotypic and molecular analysis. This lab also requires students to familiarize themselves with bioinformatics programs in which they practice analyzing sequence data of purple stem and non-purple stem alleles of the anthocyaninless genes. They then translate the sequences and discuss how the mutations found in non-purple stem alleles impact gene function. Kit uses GelGreen™ DNA stain and equips 24 students working in groups of 4. Kit also comes with free 1-year access to digital resources that support 3-dimensional instruction for NGSS. Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishable materials.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Primer/Loading Dye Mix 1 Included
W Primer/Loading Dye Mix 1 Included
pBR322/BstN1 Marker 1 Included
TE (Tris/EDTA) Buffer with RNase, 3 mL 1 Included
Wisconsin Fast Plants® Seed, P1, Non-Purple Stem Hairless 30 Included
Wisconsin Fast Plants® Seed, P2, Purple Stem Hairy 30 Included
Wisconsin Fast Plants® Seed, F2, Non-Purple Stem Hairless x Purple Stem Hairy 50 Included
Petri Dishes 25 Included
Filter Paper, 9 cm 100 Included
Edwards Buffer, 20 mL 1 Included
Pellet Pestle 15 Included
PCR Beads in 0.2-mL PCR Tubes 25 Included
Isopropanol, 10 mL 1 Included
Agarose, 4 g 1 Included
GelGreen™ Stain, 100 mL 1 Included
5 M NaCl, 8 mL 1 Included
TBE Buffer, 20x, 200 mL 1 Included
Staining Trays 6 Included
Gloves 12 Included
Carolina Science Online® Resources (1-year subscription) 1 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Micropipets and Tips, 1 µL to 1,000 µL Needed, Not Included
Microcentrifuge Tubes, 1.5 mL Needed, Not Included
Microcentrifuge for 1.5-mL Tubes Needed, Not Included
PCR Tubes, 0.2 mL or 0.5 mL Needed, Not Included
Tube Adapters for Microcentrifuge, 0.2 mL or 0.5 mL Needed, Not Included
Programmable Thermal Cycler Needed, Not Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
Container with Cracked or Crushed Ice Needed, Not Included
Water Needed, Not Included
Camera (optional) Needed, Not Included
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