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  • Genotype-Phenotype Connection Extraction, Amplification, Electrophoresis Kit with GelGreen Stain (with perishables)

Genotype-Phenotype Connection Extraction, Amplification, Electrophoresis Kit with GelGreen Stain (with perishables)

Item # 211219P Exclusive
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Looking for a great way to introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques? Students use the model microbe Halobacterium sp. NRC-1 to perform DNA extractions, PCR, and gel electrophoresis. Kit contains teacher's manual with student guide and materials for 25 DNA extractions and PCR reactions, plus materials for electrophoresis using GelGreen™.

Price

$255.00

In stock and available to ship.
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Overview

Looking for a great way to introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques? In this activity, students use the model microbe Halobacterium sp. NRC-1 to perform deoxyribonucleic acid (DNA) extractions, polymerase chain reactions (PCR), and gel electrophoresis. The activity is designed in inquiry format to allow students to observe and experience the Archaeon and extreme halophile (salt-loving) microorganism, Halobacterium sp. NRC-1, along with a stable mutant derivative, Halobacterium sp. KBT-1. Students identify the phenotypic differences between these colonies and use PCR to analyze DNA from either a mutant (KBT-1), a wild type (NRC-1), or a sectored colony, further cementing the genotype-phenotype connection.

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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What's Included

This item ships with living or perishable materials (all items). Please select a delivery date during Checkout.

Components Qty Included?
Halobacterium sp. KBT-1 (mutant)   2 Included
Halobacterium sp. NRC-1 (wild type)   3 Included
Primer-Loading Dye Mix   1 Included
1-kb Ladder/DNA Marker, 30 μL   1 Included
GelGreen, 100 μL 1 Included
5 M Sodium Chloride, 8 mL 1 Included
Sterile Water, 20 mL 2 Included
PCR Tubes Containing Ready-To-Go-Beads™ (0.2 mL capacity) 25 Included
Agarose, 2 g 1 Included
TBE Buffer, 150 mL 1 Included
Staining Trays 4 Included
Disposable Gloves 4 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Pipets Needed, Not included
Resealable Plastic Bags or Containers Needed, Not included
Permanent Fine-tip Markers Needed, Not included
Microcentrifuge (with adapters for 0.2 mL tubes) Needed, Not included
Ice Needed, Not included
Thermal Cycler Needed, Not included
Gel Electrophoresis Chambers Needed, Not included
Power Supplies Needed, Not included
Gel Trays Needed, Not included
Gel Combs Needed, Not included
Metric Ruler Needed, Not included
Microwave, Heatable Stir Plate, or Boiling Water Bath Needed, Not included
UV Transilluminator, Fluorescent White Light Box, or Blue Light Box Needed, Not included
UV Safety Goggles or Blue Light Safety Goggles or Screen Needed, Not included
Camera Needed, Not included
Household Bleach Solution (10%) or Autoclave Needed, Not included
Gloves Needed, Not included
Semliog Graph Paper Needed, Not included
Lab Coats or Aprons Needed, Not included
Vortex (optional) Needed, Not included
Stereomicroscope or Hand Lens (optional) Needed, Not included

Resources

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Sample Teacher Manuals

 

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