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DNA Barcode Amplification and Electrophoresis Kit with GelGreen® Stain (with voucher)

$241.55

Description

Advanced—For experienced high school and college classes; requires some technical skill.

  • Revised protocol saves you time and delivers robust results
  • Use DNA barcodes to identify student-collected samples of plants or animals
  • Designed, tested, and updated with the classroom in mind
  • Connect students to real-world bioinformatics
  • Identify diversity by taking taxonomy to the next level
This kit explores an emerging trend in molecular biology and brings bioinformatics to the classroom in an accessible manner. Students take a new approach to taxonomy using "DNA barcodes"—short, unique DNA sequences—to learn about the biodiversity of plants, mammals, fish, or insects. Unlock the barcodes of even more organisms by purchasing the Fungal Primer Set (item #211388, sold separately) to give students the opportunity to explore the diverse world of fungi. Using polymerase chain reaction (PCR) on specific regions of the rbcL or COI gene, students amplify their extracted DNA to create unique barcodes. These amplified sequences (amplicons) are then submitted for sequencing and can be analyzed using our custom-designed online tool, DNA Subway. Our partner, GENEWIZ, Inc., offers an educational discount for sequencing services. Kit contains enough materials for 25 DNA extractions, 25 PCR reactions, and running and staining gels with GelGreen®.

Specifications

Shipping Information or Purchase Restrictions
  • Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.
What’s Included:
  • 1 Plant rcbL Primer/Loading Dye Mix, 700 μL (voucher item)
  • 1 Fish COI Primer/Loading Dye Mix, 700 μL (voucher item)
  • 1 Insect-Mammal Primer/Loading Dye Mix, 700 μL (voucher item)
  • 1 pBR322/BstNI Markers, 0.075 μg/μL, 130 μL (voucher item)
  • 1 Silica Resin, 200 μL (voucher item)
  • 1 Wash Buffer Concentrate, 23.7 mL
  • 1 Sterile Distilled Water, 4 mL
  • 1 Agarose, 8 g
  • 1 20x TBE Buffer, 150 mL
  • 1 GelGreen® DNA Stain, 100 µL
  • 1 5M NaCl, 8 mL
  • 12 Latex Gloves
  • 6 Staining Trays
  • 1 Ethanol, 95%, 30 mL
  • 1 Mineral Oil, 5 mL
  • 25 Ready-to-Go™ PCR Beads (0.2-mL tubes)
  • 25 Plastic Pestles
  • 4 PCR 8-Tube Strips
  • 1 Teacher's Manual with Reproducible Student Guide
Needed But Not Included:
  •  Collection Tubes, Jars, or Bags
  •  Tweezers/Scissors/Scalpel
  •  Water Baths or Heat Blocks, 37° C and 65° C
  •  Micropipets and Tips, 1 to 1,000 μL
  •  Microcentrifuge for 1.5-mL Tubes
  •  Microcentrifuge Tubes, 1.5 mL
  •  Microcentrifuge Tube Racks
  •  Programmable Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  UV Transilluminator or White Light Box
  •  Permanent Markers
  •  Container with Cracked or Crushed Ice
  •  Computer with Internet Access
  •  Safety Goggles
  •  Vortexer (optional)
  •  Camera or Photo-Documentary System (optional)
  •  Smartphone or Camera with GPS (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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