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GM Food Extraction, Amplification, and Electrophoresis Kit with GelGreen™ and 0.2-mL Tubes (with voucher)

$247.55

Description

Advanced - For experienced high school and college classes; requires some basic technical skill.

Explore a modern application of genetic engineering through this exciting laboratory activity. Many commercial crops are genetically modified (GM) to include a transferred gene (transgene) for herbicide resistance. This activity investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and GM plant material (provided controls), and from food products of their choice. They use the extracted DNA as a template in 2 separate PCR reactions run under the same conditions. One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification. Each group works with a plant control and a food product. Kit includes materials for 25 PCR reactions and a voucher to request perishable materials later at your convenience. Contact us or return voucher to request delivery of perishables. Note: Order kit to arrive 1 month before performing lab.

FREE 1-year access to digital resources that support NGSS instruction included.

Specifications

Shipping Information or Purchase Restrictions
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 16 Wild-Type Soybean Seeds (voucher item)
  • 16 Roundup Ready® Soybean Seeds (voucher item)
  • 1 pBR322/BstN1 Markers, 130 µL (voucher item)
  • 1 Tris/EDTA (TE) Buffer with RNase A, 3 mL (voucher item)
  • 1 35S Primer/Loading Dye Mix, 500 µL (voucher item)
  • 1 Tubulin Primer/Loading Dye Mix, 500 µL (voucher item)
  • 1 Edwards' Buffer, 20 mL
  • 1 Isopropanol, 100%, 10 mL
  • 1 Agarose, 8 g
  • 25 PCR Tubes Containing Ready-To-Go-Beads™ (0.2-mL capacity)
  • 1 Mineral Oil, 5 mL
  • 15 Pellet Pestles
  • 25 Microcentrifuge Tubes
  • 1 TBE 20x, 150 mL
  • 1 GelGreen® Nucleic Acid Stain, 100 µL
  • 1 5 M NaCL, 8 mL
  • 12 Latex Gloves
  • 6 Staining Trays
  • 1 Teacher's Manual with Reproducible Student Guide
Needed But Not Included:
  •  Document Camera or Overhead Projector
  •  Planting Pot or Flat
  •  Potting Soil
  •  Soy or Corn Food Products
  •  Micropipets and Tips (1 to 1,000 µL)
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge (for 1.5-mL tubes)
  •  Thermal Cycler
  •  Water Bath or Heating Block
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  UV or Blue Light Transilluminator
  •  Permanent Markers
  •  Container of Crushed Ice
  •  Camera (optional)
  •  Vortexer (optional)
  •  Roundup® Herbicide (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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