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800.334.5551

GM Food Extraction and Amplification Kit with 0.2-mL Tubes (with voucher)

$206.50
(in stock)

Description

Advanced - For experienced high school and college classes; requires some basic technical skill.

Explore a modern application of genetic engineering through this exciting laboratory activity. Many commercial crops are genetically modified (GM) to include a transferred gene (transgene) for herbicide resistance. This activity investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and GM plant material (provided controls), and from food products of their choice. They use the extracted DNA as a template in 2 separate PCR reactions run under the same conditions. One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification. Each group works with a plant control and a food product. Kit includes materials for 25 PCR reactions and a voucher to request perishable materials later at your convenience. Contact us or return voucher to request delivery of perishables. Note: Order kit to arrive 1 month before performing lab.

FREE 1-year access to digital resources that support NGSS instruction included.

Specifications

What’s Included:
  • 16 Wild-Type Soybean Seeds
  • 16 Roundup Ready® Soybean Seeds
  • 1 pBR322/BstN1 Markers, 130 μL
  • 1 Tris/EDTA (TE) Buffer with RNase A, 3 mL
  • 1 35S Primer/Loading Dye Mix, 500 μL
  • 1 Tubulin Primer/Loading Dye Mix, 500 μL
  • 1 Edwards' Buffer, 20 mL
  • 1 Isopropanol, 100%, 10 mL
  • 25 PCR Tubes Containing Ready-To-Go-Beads™ (0.2 mL capacity)
  • 1 Mineral Oil, 5 mL
  • 15 Pellet Pestles
  • 25 Microcentrifuge Tubes
  • 1 Teacher's Manual and Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  •  Document Camera or Overhead Projector
  •  Planting Pot or Flat
  •  Potting Soil
  •  Soy or Corn Food Products
  •  Micropipets and Tips (1 µL to 1000 µL)
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge (for 1.5 mL tubes)
  •  Thermal Cycler
  •  Water Bath or Heating Block
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  UV Transilluminator (for ethidium bromide stain)
  •  White Light Box (for CarolinaBLU® stain)
  •  Permanent Markers
  •  Container of Crushed Ice
  •  Camera (optional)
  •  Vortexer (optional)
  •  Roundup® Herbicide (optional)
  • expand to see full list
Return Policy:

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