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Identifying the Mutation in Non-Purple Stem Wisconsin Fast Plants® Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU™ Stain and 0.2-mL Tubes (with voucher)

(in stock)


Advanced—For experienced high school and college classes; requires some technical skill.
Making a genotype-to-phenotype connection using Wisconsin Fast Plants®

This lab explores the connection between Mendelian genetics and molecular biology through the analysis of the mutation responsible for the non-purple stem trait in Wisconsin Fast Plants®. Students predict the phenotype and genotype for stem color in the F2 generation of a monohybrid cross and propose an explanation as to why the anl allele leads to a non-purple stem phenotype, whereas the ANL allele leads to the purple stem phenotype. Students finally plant F2 seeds on a monohybrid cross that will be used for phenotypic and molecular analysis. This lab also requires students to familiarize themselves with bioinformatics programs in which they practice analyzing sequence data of purple stem and non-purple stem alleles of the anthocyaninless genes. They then translate the sequences and discuss how the mutations found in non-purple stem alleles impact gene function. Kit uses CarolinaBLU® DNA stain and equips 24 students working in groups of 4. Kit also comes with free 1-year access to digital resources that support 3-dimensional instruction for NGSS. Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.


What’s Included:
  • 1 Primer/Loading Dye Mix (voucher item)
  • 1 W Primer/Loading Dye Mix (voucher item)
  • 1 pBR322/BstN1 Marker (voucher item)
  • 1 TE (Tris/EDTA) Buffer with RNase, 3 mL (voucher item)
  • 30 Wisconsin Fast Plants® Seed, P1, Non-Purple Stem Hairless
  • 30 Wisconsin Fast Plants® Seed, P2, Purple Stem Hairy
  • 50 Wisconsin Fast Plants® Seed, F2, Non-Purple Stem Hairless x Purple Stem Hairy
  • 25 Petri Dishes
  • 100 Filter Paper, 9 cm
  • 1 Edwards Buffer, 20 mL
  • 15 Pellet Pestles
  • 25 PCR Beads in 0.2-mL PCR Tubes
  • 1 Isopropanol, 10 mL
  • 1 Agarose, 4 g
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • CarolinaBLU® Final Stain, 400 mL
  • 1 TBE Buffer, 20x, 200 mL
  • 6 Staining Trays
  • 12 Gloves
  • 1 Carolina Science Online® Resources (1-year subscription)
  • 1 Teacher's Manual with Reproducible Student Guide
Needed But Not Included:
  •  Micropipets and Tips, 1 μL to 1,000 μL
  •  Microcentrifuge Tubes, 1.5 mL
  •  Microcentrifuge for 1.5-mL Tubes
  •  PCR Tubes, 0.2 mL or 0.5 mL
  •  Tube Adapters for Microcentrifuge, 0.2 mL or 0.5 mL
  •  Programmable Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Container with Cracked or Crushed Ice
  •  Water
  •  Camera (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.


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