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Identifying the Mutation in Non-Purple Stem Wisconsin Fast Plants® Extraction, Amplification, and Electrophoresis Kit with GelGreen™ Stain and 0.2-mL Tubes (with voucher)

(in stock)


Advanced—For experienced high school and college classes; requires some technical skill.

Making a genotype-to-phenotype connection using Wisconsin Fast Plants®

This lab explores the connection between Mendelian genetics and molecular biology through the analysis of the mutation responsible for the non-purple stem trait in Wisconsin Fast Plants®. Students predict the phenotype and genotype for stem color in the F2 generation of a monohybrid cross and propose an explanation as to why the anl allele leads to a non-purple stem phenotype, whereas the ANL allele leads to the purple stem phenotype. Students finally plant F2 seeds on a monohybrid cross that will be used for phenotypic and molecular analysis. This lab also requires students to familiarize themselves with bioinformatics programs in which they practice analyzing sequence data of purple stem and non-purple stem alleles of the anthocyaninless genes. They then translate the sequences and discuss how the mutations found in non-purple stem alleles impact gene function. Kit uses GelGreen® DNA stain and equips 24 students working in groups of 4. Kit also comes with free 1-year access to digital resources that support 3-dimensional instruction for NGSS. Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.


What’s Included:
  • 1 Primer/Loading Dye Mix
  • 1 W Primer/Loading Dye Mix
  • 1 pBR322/BstN1 Marker
  • 1 TE (Tris/EDTA) Buffer with RNase, 3 mL
  • 30 Wisconsin Fast Plants® Seed, P1, Non-Purple Stem Hairless
  • 30 Wisconsin Fast Plants® Seed, P2, Purple Stem Hairy
  • 50 Wisconsin Fast Plants® Seed, F2, Non-Purple Stem Hairless x Purple Stem Hairy
  • 25 Petri Dishes
  • 100 Filter Paper, 9 cm
  • 1 Edwards Buffer, 20 mL
  • 15 Pellet Pestle
  • 25 PCR Beads in 0.2-mL PCR Tubes
  • 1 Isopropanol, 10 mL
  • 1 Agarose, 4 g
  • 1 GelGreen® Stain, 100 mL
  • 1 5 M NaCl, 8 mL
  • 1 TBE Buffer, 20x, 200 mL
  • 6 Staining Trays
  • 12 Gloves
  • 1 Carolina Science Online® Resources (1-year subscription)
  • 1 Teacher's Manual with Reproducible Student Guide
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Needed But Not Included:
  •  Micropipets and Tips, 1 µL to 1,000 µL
  •  Microcentrifuge Tubes, 1.5 mL
  •  Microcentrifuge for 1.5-mL Tubes
  •  PCR Tubes, 0.2 mL or 0.5 mL
  •  Tube Adapters for Microcentrifuge, 0.2 mL or 0.5 mL
  •  Programmable Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Container with Cracked or Crushed Ice
  •  Water
  •  Camera (optional)
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Return Policy:

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