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PTC Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU™ and 0.2-mL Tubes (with perishables)

$243.00
Product Highlights
  • Make genotype-to-phenotype connections using your students’ own DNA
  • Teach the concept and importance of SNPs and their effects on phenotype
  • Connect students to real-world bioinformatics

Description

Product Details
Advanced - For experienced high school and college classes; requires some basic technical skill.

This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930s by Cold Spring Harbor Laboratory scientist Albert Blakeslee.

Students will:

  • Determine their ability to taste PTC using taste paper
  • Use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA
  • Amplify a 221-nucleotide region of the PTC taste receptor gene
  • Perform restriction digestion to differentiate the 2 alleles by gel electrophoresis
  • Determine how well the SNP genotypes actually correlate to tasting

Kit provides materials for 25 PCR reactions and restriction digests, and it includes the perishable materials when shipped.

Product Features

  • Uses CarolinaBLU® DNA stains
  • PuReTaq™ Ready-to-Go™ PCR Beads
  • Electrophoresis reagents
  • Kit equips 25 students

Specifications

Shipping Information or Purchase Restrictions
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 1 pBR322/BstNI Markers, 0.075 μg/μL, 200 μL   (perishable item)
  • HaeIII Restriction Enzyme, 40 μL   (perishable item)
  • 1 PTC Primer/Loading Dye Mix, 700 μL   (perishable item)
  • 1 Chelex® Resin, 1.5 g
  • 25 Ready-to-Go™ PCR Beads (0.2 mL tubes)
  • 1 Mineral Oil, 5 mL
  • 100 PTC Taste Strips
  • 100 Control Taste Strips
  • 1 Agarose, 10 g
  • 1 TBE Buffer, 200 mL
  • CarolinaBLU® Final Stain, 375 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • 12 Latex Gloves
  • 9 Staining Trays
  • 1 Teacher's Manual and Reproducible Student Guide
Needed But Not Included:
  •  Saline Solution, 0.9%
  •  Micropipets and Tips, 1 μL to 1000 μL
  •  Microcentrifuge Tubes, 1.5 mL
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge for 1.5 mL Tubes
  •  PCR Tubes, 0.2 or 0.5 mL
  •  Tube Adapters for Microcentrifuge, 0.2 or 0.5 mL
  •  Programmable Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Gel-staining Trays
  •  White Light Box
  •  Paper Cups
  •  Permanent Markers
  •  Container with Cracked or Crushed Ice
  •  Boiling Water Bath (optional)
  •  Camera (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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