Login or Register

800.334.5551

PTC Extraction, Amplification, and Electrophoresis Kit with Ethidium Bromide and 0.2-mL Tubes (with voucher)

$252.95
(in stock)

Description

Advanced - For experienced high school and college classes; requires some basic technical skill.

This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930s by Cold Spring Harbor Laboratory scientist Albert Blakeslee. Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene. The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles. One allele strongly correlates to the ability to taste PTC. After scoring their SNP genotypes, students determine how well the SNP genotypes actually correlate to tasting.

Kit provides materials for 25 PCR reactions and restriction digests. Kit also includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishables.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
What’s Included:
  • 1 pBR322/BstNI Markers, 0.075 μg/μL, 200 μL
  • HaeIII Restriction Enzyme, 40 μL
  • 1 PTC Primer/Loading Dye Mix, 700 μL
  • 1 Chelex® Resin, 1.5 g
  • 25 Ready-to-Go™ PCR Beads (0.2 mL tubes)
  • 1 Mineral Oil, 5 mL
  • 100 PTC Taste Strips
  • 100 Control Taste Strips
  • 1 Agarose, 10 g
  • 1 TBE Buffer, 200 mL
  • 1 Ethidium Bromide, 1 μg/mL, 200 mL
  • 12 Latex Gloves
  • 9 Staining Trays
  • 1 Teacher's Manual and Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  •  Saline Solution, 0.9%
  •  Micropipets and Tips, 1 μL to 1000 μL
  •  Microcentrifuge Tubes, 1.5 mL
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge for 1.5 mL Tubes
  •  PCR Tubes, 0.2 or 0.5 mL
  •  Tube Adapters for Microcentrifuge, 0.2 or 0.5 mL
  •  Programmable Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Gel-staining Trays
  •  UV Transilluminator
  •  Paper Cups
  •  Permanent Markers
  •  Contrainer with Cracked or Crushed Ice
  •  Boiling Water Bath (optional)
  •  Camera (optional)
  • expand to see full list
Return Policy:

This product is backed by our 100% Satisfaction Guarantee. If for any reason you are not satisfied with any item, you may return it for a replacement, a refund, or credit. Read more about our Return and Exchange Policy  »

Reviews

Questions & Answers