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Assay for Antibiotic Resistance Kit

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This lab was developed in cooperation with the authors of DNA Science: A First Course-2nd Edition (# 212209) to teach your students about a serious problem facing society-bacterial antibiotic resistance-while introducing them to basic enzymology assays. Students collect the supernatant from ampicillin-resistant bacterial cultures and perform a simple colorimetric assay to detect the activity of β-lactamase, the enzyme conferring ampicillin resistance.

 
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Intermediate - Easy to perform; requires some background knowledge.

This lab was developed in cooperation with the authors of DNA Science: A First Course-2nd Edition (# 212209) to teach your students about a serious problem facing society-bacterial antibiotic resistance-while introducing them to basic enzymology assays. Students collect the supernatant from ampicillin-resistant bacterial cultures and perform a simple colorimetric assay to detect the activity of β-lactamase, the enzyme conferring ampicillin resistance. Kit contains enough for 6 teams of students.

** Kit and perishable components will ship separately and may arrive on different days.

 
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