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Carolina LabSheets

  • Introduction to Prokaryotes: Bacteria What are bacteria? In this introductory lab students make smears of bacteria on microscope slides from pure cultures, perform a simple stain, and observe their stained slides under a microscope View »
  • Introduction to Prokaryotes: Archaea In this lab, students are introduced to Halobacterium, a representative of the Archaea, one of the three domains of life (along with Bacteria and Eukarya). View »
  • Introduction to Sterile Technique In this lab, students practice transfer of a bacterial culture using sterile technique. View »
  • Introduction to Fungi This lab introduces students to the Kingdom Fungi through study of two phyla, the zygomycetes and basidiomycetes. View »
  • Isolating Bacteria from a Mixture How does a microbiologist isolate bacteria from a sample? In this lab students practice streaking a plate to isolate a single species of bacterium from a known mixture. View »
  • Wisconsin Fast Plants® and Product Testing In this inquiry activity, students conduct experiments to determine if a commercial product effects the germination of Wisconsin Fast Plants® seed. In doing so they practice basic science skills including collecting and graphing data, interpreting data, and doing serial dilutions. View »
  • Introduction to Algae In this lab, students observe a variety of algae and record basic observations of them. View »
  • Introduction to Protista: Euglena Euglena was once studied as a simple animal by zoologists and as a simple plant by botanists. How does this protist combine some characteristics of animals with the plant-like ability to photosynthesize? View »
  • Carolina LabSheets™: Protista: Key to Algae Mixtures What are algae? In this lab students observe a variety of algae and record their characteristics. View »
  • Introduction to Prokaryotes: Cyanobacteria In this lab students observe two examples of cyanobacteria and make a simple comparison to a eukaryotic green alga. View »
  • Bacterial Growth on MacConkey Agar In this lab students culture three bacteria on nutrient agar and MacConkey agar and record the results. View »
  • Introduction to Protista: Paramecium Complexity in a single cell. In this lab students observe Paramecium as an example of a complex ciliated protist. View »
  • Vinegar Eels In this lab, students observe vinegar eels. View »
  • Two-Point Linkage with Drosophila In this lab students explore the genetic consequences of gene linkage using an F1 cross of Drosophila. View »
  • Three-Point Linkage with Drosophila In this lab students investigate the effects of gene linkage and crossing-over on the inheritance of three traits of Drosophila. They use the data they collect to construct a linkage map of the gene loci. View »
  • Testing for Segregation of Alleles In this lab, students test corn pollen for evidence of the segregation of alleles. View »
  • Survey of Protista Protists are highly diverse. In this lab students are introduced to this diversity through observing a selection of protists and recording their characteristics. View »
  • Carolina LabSheets™: Seed Germination In this inquiry activity students in grades 5-10 design and conduct an experiment to investigate factors that may affect seed germination. View »
  • Pill Bug Behavior This activity is an introduction to animal behavior. Students conduct an experiment to test the response of pill bugs (Armadillidium vulgare) to conditions of low and high humidity. View »
  • Meiosis and Genetics In this lab, students investigate how the events of meiosis relate to Mendelian genetics. View »
  • Lumbriculus: Contraction Rate of the Dorsal Blood Vessel In this introductory physiology lab, students determine the contraction rate of the dorsal blood vessel of the blackworm Lumbriculus. Students then design and conduct an experiment to determine the effect of temperature change or chemical exposure on the contraction rate. View »
  • Investigating a Phenotype In this activity, students investigate a phenotype that is not so conspicuous, and they perform a simple chemical test to reveal the underlying basis of the phenotype. View »
  • Investigating Seed Germination The story of how a seed becomes a plant is fascinating to young students. These activities for grades K-4 will help your students explore the process of germination, seed structure, and early seedling growth. View »
  • Investigating Hatching of Brine Shrimp Eggs In this lab students study the hatching of brine shrimp eggs and design an experiment to see what factors influence hatching. View »
  • Introductory Genetics with Drosophila Use this LabSheet with a variety of our Drosophila cultures and sets for introductory labs covering monohybrid, dihybrid, and sex-linked crosses. View »
  • Introduction to Protista: Amoeba What characteristics do protists share with animals? In this lab students observe Amoeba proteus to find out. View »
  • Introduction to Planaria In this lab, students examine the anatomy and behavior of the planarian, a simple animal with bilateral symmetry. View »
  • Introduction to Lichens In this lab students observe basic lichen types (crustose, foliose, fruticose and fruticose pendant) and discover that lichens are composite organisms, consisting of fungal and algal components. View »
  • Introduction to Invertebrates In this lab, students study representatives of several invertebrate groups. View »
  • Introduction to Hydra This lab introduces students to Phylum Cnidaria. The activity also serves as an introduction to animals. View »
  • Introduction to Drosophila: Phenotypes In this lab students compare phenotypes of mutant Drosophila to wild type. The activity is intended as a student’s first experience working with Drosophila. View »
  • Introduction to Blood Types In this lab, students are introduced to the human ABO blood groups. View »
  • Carolina LabSheets™: Introduction to Ascomycetes This lab introduces students to the variation found in the fungal phylum Ascomycota. Students examine four representatives: Anthracobia muelleri, Eurotium chevalieri, Schizosaccharomyces octosporus, and Sordaria fimicola. View »
  • Genetics with Drosophila F1 Crosses: Sex-Linkage Drosophila genetics labs without the the need to select virgin female flies for crosses. We ship F1 flies so your students need only to set them up in fresh vials to produce an F2. View »
  • Genetics with Drosophila F1 Crosses Drosophila genetics labs without the the need to select virgin female flies for crosses. We ship F1 flies so your students need only to set them up in fresh vials to produce an F2. View »
  • Carolina LabSheets™: Gene Interaction with Drosophila How do genes interact in the inheritance of traits? Find out in this intriguing study of the inheritance of eye color in Drosophila. View »
  • Embryology with Rhabditis In this lab students observe cleavage of fertilized eggs of female Rhabditis. View »
  • Daphnia Heart Rate In this introductory physiology lab, students determine the heart rate of Daphnia magna and then test the effect of changing temperature on the heart rate. View »
  • Constructing a Cladogram with Hydra, Planaria, and Daphnia In this lab students compare the body structure and functions of three invertebrates to that of a vertebrate. They use their data to construct a cladogram. View »
  • Basic Mold Showplate Set This introduction to fungi focuses on the production of asexual spores by three fungi: Aspergillus, Penicillium, and Rhizopus. View »
  • Animal or Not? In this lab students compare an animal to an animal-like protist, or protozoan. View »
  • What’s the Genotype? In this lab students are introduced to the testcross and its use in revealing whether an organism is homozygous or heterozygous for a trait. View »
  • Investigating a Lethal Trait In this lab, students investigate a lethal trait, albinism, using corn seedlings. View »
  • Introduction to Phenotypes with Wisconsin Fast Plants® In this lab, students germinate seeds of Wisconsin Fast Plants and examine the plants that grow from them for differences in appearance. This introductory-level activity can be used as a pre-lab to a unit on Mendelian genetics, and assumes that students are familiar with the terms genotype, phenotype, and allele. View »
  • Dealing with Data In this introductory lab, students collect data and then devise methods to organize and display the data to give it more meaning. After brainstorming and evaluating their methodologies, they graph the data and perform a written analysis of their results. View »
  • Biodiversity of Soil Animals In this lab, students use a Berlese funnel to take samples of soil-dwelling animals, and then compare the numbers and kinds of organisms collected from different habitats. View »
  • Bacterial Motility In this lab, students perform two exercises that investigate microbial motility. One is on a microscopic level, using the “hanging drop” slide preparation method to directly observe motile cells. The second exercise is on a macroscopic level and involves inoculating motility test media. View »
  • Carolina LabSheets™: Bacterial Hydrolysis of Starch In this lab, students culture two bacteria on potato dextrose agar, which contains starch. When grown on potato dextrose agar, bacteria that can secrete amylase—an enzyme that hydrolyzes starch—create a zone around their colonies in which starch is absent. When the agar is flooded with iodine solution, most of the plate stains dark blue–black, but clear areas are left around colonies that secrete amylase. View »
  • Bacterial Hydrolysis of Lipids In this lab, students culture two species of bacteria on agar medium that contains an emulsion of plant oils and the dye, sprit blue, which forms a complex with the triglycerides of the oils and gives an opaque blue color to the agar. Colonies of bacteria that can secrete lipase, an enzyme that hydrolyzes triglycerides, develop a light area or “halo” in the surrounding medium, due to the diffusion of lipase into the medium and the resultant breakdown of the oil/spirit blue complex. View »
  • Carolina LabSheets™: Bacterial Hydrolysis of Casein In this lab, students culture two bacteria on skim milk agar. The protein casein gives milk its white color. Some bacteria secrete protease enzymes that can hydrolyze casein. When these bacteria are grown on skim milk agar a clear area develops around the colonies, indicating that casein has been hydrolyzed into its component amino acids. View »
  • Bacteria: The Indole Test In this lab students perform a differential test to distinguish bacteria based on the production of indole. Bacteria are grown on media containing tryptophan and then treated with Kovac’s reagent. If they possess the enzyme tryptophanase, the bacteria can metabolize tryptophan into indole, pyruvic acid, and ammonia. View »
  • Carolina LabSheets™: Bacteria: The Citrate Test In this lab students perform a test on two different bacteria to determine whether the organisms can use citric acid as their sole carbon source. The citrate test is often performed as part of the IMViC (Indole, Methyl Red, Voges-Proskauer, and Citrate) series of tests used to differentiate common species of enteric bacteria. View »
  • Introduction to Photosynthesis Using Coleus Plants The simple exercises that students perform in this lab demonstrate that plants produce glucose through photosynthesis, that plants convert at least some of the glucose to starch, and that sunlight is a critical factor in photosynthesis. View »
  • Linkage Determination with Drosophila In this lab students use the Drosophila stock Curly/Plum; Dichaete/Stubble to determine which chromosome an “unknown” mutation is located upon. They also determine whether the unknown mutation is dominant or recessive, and whether it is autosomal or sex-linked. View »
  • Carolina LabSheets™: Seedling Genetics (Corn) Students study F2 seedlings to discover how a dwarf phenotype is inherited in corn. They determine that the allele for tall height exhibits complete dominance to the allele for dwarf, that the seedlings are segregating in a ratio of 3 tall to 1 dwarf, and that the parent plant was tall and heterozygous. View »
  • Carolina LabSheets™: Seedling Genetics (Sorghum) Students study F2 seedlings to discover how a color phenotype is inherited in sorghum. They can study a cross involving green and albino plants or, alternatively, a cross involving red and green plants. View »
  • Carolina LabSheets™: Seedling Genetics (Soybean or Tomato) From a study of either soybean seedlings or tomato seedlings, students determine that the color alleles involved exhibit incomplete dominance, that the seedlings are segregating in a ratio of 1 green:2 yellow-green:1 yellow, and that the parent plant was yellow-green and heterozygous. View »
  • Observing Plant Cells In this lab students observe Elodea leaves under magnification. They will see cell walls and chloroplasts. From the movement of chloroplasts they will infer that cyclosis, or protoplasmic streaming, is occurring. They also will observe that most chloroplasts are pressed tightly against the cell wall and should infer from this that much of the cell is occupied by a vacuole. View »
  • Introduction to Feeding Preferences in Caenorhabditis elegans The nonparasitic soil nematode Caenorhabditis elegans has served as a model organism for research scientists since the 1960s. This lab introduces students to the organism and provides them with the tools necessary to perform simple experiments. View »
  • Make a Hay Infusion Hay infusions are widely used as a source of microorganisms for studying decomposition, fermentation, and disease. And preparing one is easy--simply soak fresh or dried plant material in water. View »
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