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Life Science

  • Bacteria: The Citrate Test In this lab students perform a test on two different bacteria to determine whether the organisms can use citric acid as their sole carbon source. The citrate test is often performed as part of the IMViC (Indole, Methyl Red, Voges-Proskauer, and Citrate) series of tests used to differentiate common species of enteric bacteria. View »
  • Introduction to Sterile Technique In this lab, students practice transfer of a bacterial culture using sterile technique. View »
  • Biodiversity of Soil Animals In this lab, students use a Berlese funnel to take samples of soil-dwelling animals, and then compare the numbers and kinds of organisms collected from different habitats. View »
  • Bacterial Hydrolysis of Starch In this lab, students culture two bacteria on potato dextrose agar, which contains starch. When grown on potato dextrose agar, bacteria that can secrete amylase—an enzyme that hydrolyzes starch—create a zone around their colonies in which starch is absent. When the agar is flooded with iodine solution, most of the plate stains dark blue–black, but clear areas are left around colonies that secrete amylase. View »
  • Introduction to Prokaryotes: Cyanobacteria In this lab students observe two examples of cyanobacteria and make a simple comparison to a eukaryotic green alga. View »
  • Carolina's Recommended Reading for AP® Biology Teaching AP® Biology this year? Go beyond the textbook and take students on an exploration of key concepts with these teacher-tested and recommended books. View »
  • Bacteria: The Indole Test In this lab students perform a differential test to distinguish bacteria based on the production of indole. Bacteria are grown on media containing tryptophan and then treated with Kovac’s reagent. If they possess the enzyme tryptophanase, the bacteria can metabolize tryptophan into indole, pyruvic acid, and ammonia. View »
  • Infographic: What's Your Blood Type? Use this exciting infographic as a teaching aid for your classes as your students discover the ABO/Rh blood groups. View »
  • Plant Biodiversity Field Activity This field activity introduces students to biodiversity through the study of plants and animals in a designated area. But here’s the twist—instead of using a quadrat to designate the area of study, students use a Hula Hoop® instead. View »
  • Bacterial Hydrolysis of Lipids In this lab, students culture two species of bacteria on agar medium that contains an emulsion of plant oils and the dye, sprit blue, which forms a complex with the triglycerides of the oils and gives an opaque blue color to the agar. Colonies of bacteria that can secrete lipase, an enzyme that hydrolyzes triglycerides, develop a light area or “halo” in the surrounding medium, due to the diffusion of lipase into the medium and the resultant breakdown of the oil/spirit blue complex. View »
  • Carolina Quick Tips®: Next Top Model (Organism) Students seem to consistently struggle to understand Punnett squares and grasp Mendel’s laws. Bringing live organisms into your View »
  • Introduction to Prokaryotes: Bacteria What are bacteria? In this introductory lab students make smears of bacteria on microscope slides from pure cultures, perform a simple stain, and observe their stained slides under a microscope View »
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