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Life Science

  • Bacterial Hydrolysis of Lipids In this lab, students culture two species of bacteria on agar medium that contains an emulsion of plant oils and the dye, sprit blue, which forms a complex with the triglycerides of the oils and gives an opaque blue color to the agar. Colonies of bacteria that can secrete lipase, an enzyme that hydrolyzes triglycerides, develop a light area or “halo” in the surrounding medium, due to the diffusion of lipase into the medium and the resultant breakdown of the oil/spirit blue complex. View »
  • Introduction to Prokaryotes: Bacteria What are bacteria? In this introductory lab students make smears of bacteria on microscope slides from pure cultures, perform a simple stain, and observe their stained slides under a microscope View »
  • Make a Hay Infusion Hay infusions are widely used as a source of microorganisms for studying decomposition, fermentation, and disease. And preparing one is easy--simply soak fresh or dried plant material in water. View »
  • Bacterial Growth on MacConkey Agar In this lab students culture three bacteria on nutrient agar and MacConkey agar and record the results. View »
  • Next Generation Dissections Dissection can and should be part of your implementation of 3-dimensional learning. Carolina can help you make this transition with updated kits and resources. View »
  • Introduction to Prokaryotes: Cyanobacteria In this lab students observe two examples of cyanobacteria and make a simple comparison to a eukaryotic green alga. View »
  • Constructing and Testing a Dichotomous Key Model for Fruit This investigation requires students to create and test a model for fruit classification. Use it as an introductory inquiry into taxonomy for life science classes or as an NGSS Dimension 1 Practice in life science, botany, or agricultural science classes. View »
  • Bacteria: The Indole Test In this lab students perform a differential test to distinguish bacteria based on the production of indole. Bacteria are grown on media containing tryptophan and then treated with Kovac’s reagent. If they possess the enzyme tryptophanase, the bacteria can metabolize tryptophan into indole, pyruvic acid, and ammonia. View »
  • Studying Genomes in the Madagascar Forest Using miniPCR® When it’s difficult to bring DNA samples to a lab, why not bring the lab to the samples? Find out how a portable thermal cycler is changing the way a biological anthropologist performs her research. View »
  • Debunking the 4° C Polymerase Chain Reaction (PCR) Myth Do samples need to be refrigerated immediately after PCR? No. You can leave them at room temperature overnight—and much longer! The founders of miniPCR® explore why. View »
  • Constructing a Cladogram with Hydra, Planaria, and Daphnia In this lab students compare the body structure and functions of three invertebrates to that of a vertebrate. They use their data to construct a cladogram. View »
  • Exploring Monocots and Dicots Angiosperms, or flowering plants, can be classified into 2 groups—monocots and dicots. This infographic illustrates key differences. View »
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