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Life Science

  • Debunking the 4° C Polymerase Chain Reaction (PCR) Myth Do samples need to be refrigerated immediately after PCR? No. You can leave them at room temperature overnight—and much longer! The founders of miniPCR® explore why. View »
  • Introduction to Prokaryotes: Bacteria What are bacteria? In this introductory lab students make smears of bacteria on microscope slides from pure cultures, perform a simple stain, and observe their stained slides under a microscope View »
  • Introduction to Sterile Technique In this lab, students practice transfer of a bacterial culture using sterile technique. View »
  • Bacteria: The Indole Test In this lab students perform a differential test to distinguish bacteria based on the production of indole. Bacteria are grown on media containing tryptophan and then treated with Kovac’s reagent. If they possess the enzyme tryptophanase, the bacteria can metabolize tryptophan into indole, pyruvic acid, and ammonia. View »
  • Isolating Bacteria from a Mixture How does a microbiologist isolate bacteria from a sample? In this lab students practice streaking a plate to isolate a single species of bacterium from a known mixture. View »
  • Bacterial Growth on MacConkey Agar In this lab students culture three bacteria on nutrient agar and MacConkey agar and record the results. View »
  • Bringing Real-World STEM Experiences to the Classroom by using miniPCR For schools grappling with budget constraints, biotech equipment costs can make it difficult to give students true-to-life learning experiences. One biology teacher has found a new resource that makes real-world instruction more affordable. View »
  • Introduction to Prokaryotes: Cyanobacteria In this lab students observe two examples of cyanobacteria and make a simple comparison to a eukaryotic green alga. View »
  • Bacterial Hydrolysis of Lipids In this lab, students culture two species of bacteria on agar medium that contains an emulsion of plant oils and the dye, sprit blue, which forms a complex with the triglycerides of the oils and gives an opaque blue color to the agar. Colonies of bacteria that can secrete lipase, an enzyme that hydrolyzes triglycerides, develop a light area or “halo” in the surrounding medium, due to the diffusion of lipase into the medium and the resultant breakdown of the oil/spirit blue complex. View »
  • Bacterial Hydrolysis of Casein In this lab, students culture two bacteria on skim milk agar. The protein casein gives milk its white color. Some bacteria secrete protease enzymes that can hydrolyze casein. When these bacteria are grown on skim milk agar a clear area develops around the colonies, indicating that casein has been hydrolyzed into its component amino acids. View »
  • Bacterial Motility In this lab, students perform two exercises that investigate microbial motility. One is on a microscopic level, using the “hanging drop” slide preparation method to directly observe motile cells. The second exercise is on a macroscopic level and involves inoculating motility test media. View »
  • Studying Genomes in the Madagascar Forest Using miniPCR® When it’s difficult to bring DNA samples to a lab, why not bring the lab to the samples? Find out how a portable thermal cycler is changing the way a biological anthropologist performs her research. View »
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