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Bacteria: The Indole TestIn this lab students perform a differential test to distinguish bacteria based on the production of indole. Bacteria are grown on media containing tryptophan and then treated with Kovac’s reagent. If they possess the enzyme tryptophanase, the bacteria can metabolize tryptophan into indole, pyruvic acid, and ammonia. View »
Bacterial MotilityIn this lab, students perform two exercises that investigate microbial motility. One is on a microscopic level, using the “hanging drop” slide preparation method to directly observe motile cells. The second exercise is on a macroscopic level and involves inoculating motility test media. View »
Bacteria: The Citrate TestIn this lab students perform a test on two different bacteria to determine whether the organisms can use citric acid as their sole carbon source. The citrate test is often performed as part of the IMViC (Indole, Methyl Red, Voges-Proskauer, and Citrate) series of tests used to differentiate common species of enteric bacteria. View »
Biodiversity of Soil AnimalsIn this lab, students use a Berlese funnel to take samples of soil-dwelling animals, and then compare the numbers and kinds of organisms collected from different habitats. View »
Bacterial Hydrolysis of StarchIn this lab, students culture two bacteria on potato dextrose agar, which contains starch. When grown on potato dextrose agar, bacteria that can secrete amylase—an enzyme that hydrolyzes starch—create a zone around their colonies in which starch is absent. When the agar is flooded with iodine solution, most of the plate stains dark blue–black, but clear areas are left around colonies that secrete amylase. View »
Introduction to Feeding Preferences in Caenorhabditis elegansThe nonparasitic soil nematode Caenorhabditis elegans has served as a model organism for research scientists since the 1960s. This lab introduces students to the organism and provides them with the tools necessary to perform simple experiments. View »
Plant Biodiversity Field ActivityThis field activity introduces students to biodiversity through the study of plants and animals in a designated area. But here’s the twist—instead of using a quadrat to designate the area of study, students use a Hula Hoop® instead. View »