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Life Science

  • Bacterial Hydrolysis of Starch In this lab, students culture two bacteria on potato dextrose agar, which contains starch. When grown on potato dextrose agar, bacteria that can secrete amylase—an enzyme that hydrolyzes starch—create a zone around their colonies in which starch is absent. When the agar is flooded with iodine solution, most of the plate stains dark blue–black, but clear areas are left around colonies that secrete amylase. View »
  • Bacterial Motility In this lab, students perform two exercises that investigate microbial motility. One is on a microscopic level, using the “hanging drop” slide preparation method to directly observe motile cells. The second exercise is on a macroscopic level and involves inoculating motility test media. View »
  • Make a Hay Infusion Hay infusions are widely used as a source of microorganisms for studying decomposition, fermentation, and disease. And preparing one is easy--simply soak fresh or dried plant material in water. View »
  • Tied Up in Protein Synthesis (or Lost in Translation): A Kinesthetic and Inquiry-Based Approach to Teaching the Central Dogma of Biology In this exercise, students tie Windsor knots to create a translation product from mRNA instructions—a protein “necktie.” Your classroom is the nucleus, and the hallway is cytoplasm. View »
  • Science Notebook: An Owl Pellet Inquiry This introductory owl pellet dissection is a springboard for teaching the techniques of using a science notebook while having students engage in the scientific practices. View »
  • Bacteria: The Indole Test In this lab students perform a differential test to distinguish bacteria based on the production of indole. Bacteria are grown on media containing tryptophan and then treated with Kovac’s reagent. If they possess the enzyme tryptophanase, the bacteria can metabolize tryptophan into indole, pyruvic acid, and ammonia. View »
  • Introduction to Prokaryotes: Bacteria What are bacteria? In this introductory lab students make smears of bacteria on microscope slides from pure cultures, perform a simple stain, and observe their stained slides under a microscope View »
  • Bacterial Growth on MacConkey Agar In this lab students culture three bacteria on nutrient agar and MacConkey agar and record the results. View »
  • Biodiversity of Soil Animals In this lab, students use a Berlese funnel to take samples of soil-dwelling animals, and then compare the numbers and kinds of organisms collected from different habitats. View »
  • Introduction to Prokaryotes: Archaea In this lab, students are introduced to Halobacterium, a representative of the Archaea, one of the three domains of life (along with Bacteria and Eukarya). View »
  • Invertebrate Biodiversity and Abiotic Factors Understanding the relationships between biotic diversity and abiotic factors in an ecosystem can be a difficult task. Using soil invertebrates, students can identify both the number of species present in a soil sample and the number of individuals within a species. View »
  • Snakeheads Invade the United States Teach your students about the challenges nonnative species pose by focusing on a current example, the northern snakehead fish. Native to China, Russia, and Korea, this fish has established breeding populations in several states. Find out how this might have happened and what scientists are doing about it. View »
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