Login or Register

800.334.5551 Live Chat

BioBuilder® Golden Bread PCR Kit (with prepaid coupon)

Item # 217008 Online Only This product is not available in our print catalog. Exclusive This product is exclusive to Carolina Biological Supply.

Advanced—For experienced high school and college classes; requires some technical skill.
This biology lab explores the engineering of a yeast that's genetically modified to produce ß-carotene. Use PCR to test the metabolic pathway of modified Saccharomyces cerevisiae as part of the engineering design-build-test pathway and investigate how to make it possible for users to bake vitamin A-enriched loaves of bread.

Price

$137.00

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

  • Introduce bio-engineering concepts
  • Teach synthetic biology and microbiology techniques
  • Allow students to experience real-world connections

This biology lab explores the engineering of a yeast that's genetically modified to produce ß-carotene. In the body, ß-carotene is converted to vitamin A, which is crucial for vision, the immune system, and other biological functions. Use PCR to test the metabolic pathway of modified Saccharomyces cerevisiae as part of the engineering design-build-test pathway and investigate how to make it possible for users to bake vitamin A-enriched loaves of bread. Kit includes enough materials for 24 students working in groups of 4.

Students will perform:

  • Cell culture of the modified yeast on petri dishes
  • DNA extraction from yeast colonies
  • PCR to amplify genes from yeast
  • Electrophoresis to visualize results (electrophoresis materials are included in the item #217009 BioBuilder® Golden Bread PCR Electrophoresis Pack)

This lab may be performed on its own or in conjunction with the BioBuilder® Golden Bread Transformation Kit (items #217006 and #217006P). PCR could be used to test for gene content of different colored colonies before and after transformation. The transformation kit inserts a second copy of one of the enzymes needed in the biosynthetic pathway of ß-carotene, scientifically addressing the importance of genetic redundancy when engineering synthetic systems.

Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishable materials.

  • This item is only available from Carolina Biological Supply Company.
  • This item is only available at Carolina.com.

Product Families Featuring This Item:

 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
VitaYeast Culture 1 Included
Positive Control 1 Included
DNA Primer Mix 1 Included
Petri Dishes 25 Included
Inoculating Needles 30 Included
Microcentrifuge Tubes 30 Included
Ready-to-Go PCR Beads 30 Included
Melt & Pour YED Media, 350 mL 1 Included
X-Tract™ Buffer 1 Included
Sterile Water, 1 mL 1 Included
BioBuilder® Instruction Card 1 Included

Product Support