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Glow-in-the-Dark Transformation Kit

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$28.00 - $129.50 View Details

Bacteria that produce light are very common in the ocean. One luminescent bacterium is Vibrio fischeri. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.

 
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Overview

Intermediate - Easy to perform; requires some background knowledge.

Bacteria that produce light are very common in the ocean. One luminescent bacterium is Vibrio fischeri. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.

Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.

** Kit and perishable components will ship separately and may arrive on different days.

 

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Ratings & Reviews

Rated 1 out of 5 by from Storage error & transformation didn't work First issue: the box with perishables was labeled "store in freezer" and so it got put there, and it froze our E. coli. Second issue: with different E. coli, the transformation simply didn't work. We didn't have growth on any LB/amp +plates, so no glow in the dark bacteria. Pretty disappointed students (as well as teachers). Never ordering this one again.
Date published: 2018-04-12
Rated 5 out of 5 by from Worked like a charm Simple and effective! Very minimal setup required. Very small volumes were difficult to deliver, but the procedure worked perfectly despite students' obvious errors. Transformations were successful in 3 of 4 groups, and rates were high in 2 of them. Results impressed even my most advanced students.
Date published: 2016-05-01
Rated 5 out of 5 by from Glow in the dark transformation kit Works great every time and the students love it when they see what they have done.
Date published: 2015-12-11
Rated 4 out of 5 by from Fairly straight forward, average results This kit was used for an AP Biology class. Students had easy time with procedure, and the results were as expected.
Date published: 2014-12-11
Rated 4 out of 5 by from The kit is perfect; this isn't my favorite plasmid. The kit works great--the students love knowing that they genetically transformed something. Although my students had successful transformation (colonies grew in the presence of antibiotic), none of ours showed any signs of bioluminscence. I tried incubating different plates at 37 deg and at 30 deg (conflicting information in the instructions), but still none of them glowed in the dark. Overall, my students were more excited about the pGREEN results than this one, since we had no glowing. A good tip to keep in mind is to be conservative in heat-shocking the bacteria. The instructions say to keep the water bath at 42 deg. I've had great results with 40-41, but killed almost all of the bacteria at 43 (luckily a few survived, so I just incubated them twice as long and the students could still see that it worked). If you're new to this lab, make sure not to schedule your perishables delivery too early--the antibiotic (ampicillin) can break down--and the instructions are clear that you can't prepare the growth plates more than 10 days in advance.
Date published: 2014-11-19
Rated 5 out of 5 by from Students love glowing bacteria!! This lab is really engaging for students. They love it when their bacteria glow in the dark - especially the somewhat disengaged students. I've tied it into a whole unit on GMOs and the students get really excited about the idea that they are going to genetically modify an organism. Make sure you pay attention to the part where it says to incubate the transformed bacteria at room temperature! They don't glow at all if incubated at 37C. They also will lose their glow if they're refrigerated or if they're allowed to overgrow.
Date published: 2014-11-19
Rated 5 out of 5 by from Great Instructions, student questions, and worked everytime! This is a great kit all around. The supplies were efficient and worked well, setup was simple, protocol instructions easy to follow, student questions, and most important it worked flawlessly through many classes and level of students.
Date published: 2014-04-02
Rated 5 out of 5 by from Great for AP Bio A great kit! This is the fourth time I have used it. The directions are clear, it is easy to use, and it always works. And the students have something really interesting to look forward to. When the incubation period is over and the pertri dish is taken to a dark room, they always exclaim "wow!" when they see the e coli colonies glowing in the dark. Helps motivate them to understand the biological concepts behind transformation.
Date published: 2009-09-10
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