Purification of Green Fluorescent Protein Kits | Carolina.com

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Purification of Green Fluorescent Protein Kits

6 Items Exclusive
Purification of Green Fluorescent Protein Kits is rated 4.2 out of 5 by 14.
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$56.00 - $129.50 View Details

This kit is great for teaching:

  • The molecular basis of heredity
  • The relationship between genes and proteins
  • How E. coli is used to engineer proteins useful in medicine and manufacturing
  • Three techniques - transformation, protein purification, and protein gel electrophoresis, commonly used in biological research and its areas of application

 
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Intermediate/Advanced - Modules 1 and 2 require some background knowledge. Module 3 is for more experienced classes and requires some technical skill.

This kit is great for teaching:

  • The molecular basis of heredity
  • The relationship between genes and proteins
  • How E. coli is used to engineer proteins useful in medicine and manufacturing
  • Three techniques - transformation, protein purification, and protein gel electrophoresis, commonly used in biological research and its areas of application

Note: Module 1 contains a coupon for prepaid delivery of perishable items. Contact us or return the coupon to request delivery of perishable material. If you need perishables to arrive with kit, please specify when ordering.

 
 
 
 
Rated 5 out of 5 by from Excellent Transformation Success! I've bought this kit many times and noticed that this year the plasmid concentration was doubled by Carolina to increase the likelihood of success and WOW!! We had tons of colonies and the kids were really excited.
Date published: 2018-04-10
Rated 4 out of 5 by from Purification works with one modification I used to have problems with the lysis step, but we tried incubating at 37C for 15 minutes with lots of success.
Date published: 2016-02-18
Rated 5 out of 5 by from Really student-proof lab This transformation lab has success built in. The reagents and instructions are truly student-proof - there will be transformants so long as the students are truly careful. Transformation efficiencies are good and the students enjoy the fluorescent results.
Date published: 2015-11-04
Rated 2 out of 5 by from Items missing When this kit was ordered, it was missing a few things, the LB broth, directions, etc. We did get the plates, the pGreen, and the Ecoli. This was for student use for a project, and it was very frustrating for both of us that all of the components were not included.
Date published: 2015-04-09
Rated 3 out of 5 by from This product requires previous knowledge There are no instructions for the transformation included. I was able to fin some online but it wasn't for this specific kit. I also was unaware that perishables had to be ordered after the kit arrived and all of my organisms that I planned to feed this transformed bacteria for my experiment died.
Date published: 2015-03-24
Rated 4 out of 5 by from easy to use with consistent results We use this product every year, and every time we get consistent results, with a high transformation efficiency. The lab is easy to set up. The directions are straightforward and easy to follow.
Date published: 2014-12-12
Rated 1 out of 5 by from poor results The last two years that I have done this lab I'd had really poor results. I have followed all the recommendations as far as age of the bacteria, etc. and very few groups have had positive results.
Date published: 2014-12-11
Rated 5 out of 5 by from Exciting lab for engaging students! This kit has everything you need to do bacterial transformation with a plasmid. I've tried a few plasmids with my AP Biology students, and this one consistently gives very clean results. It's also my students' favorite plasmid! One tip is to be conservative in heat-shocking the bacteria. The instructions say to use a 42 deg. water bath. I've had great results if the water bath was 40-41 but killed most of the bacteria if it was 43 (luckily a few survived, so I just incubated them twice as long and the students could still see that it worked). Make sure you have a UV light source (blacklight), which is not included. The bacteria fluoresce green when successfully transformed, and the students get very excited about their "glowy" bacteria. The transformed colonies will keep fluorescing for several days after transformation, so I was able to bring it to a school showcase a few days later--it made a great impression on prospective students!
Date published: 2014-11-19
  • y_2018, m_11, d_19, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_13
  • loc_en_US, sid_FAM_211073, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_carolina
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 5ms
  • REVIEWS, PRODUCT