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  • Genetic Complementation and Recombination Spot Test Kit (with Prepaid Coupon)

Genetic Complementation and Recombination Spot Test Kit (with Prepaid Coupon)

Item # 124550
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All materials and instructions for (1) conducting complementation (cis-trans) spot tests to place unknown T4rll mutations in either cistron A or B, and for (2) conducting recombination spot tests to determine by deletion mapping the A-segments within which the rll mutations occur.

Price

$229.95

In stock and available to ship.
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Overview

All materials and instructions for (1) conducting complementation (cis-trans) spot tests to place unknown T4rll mutations in either cistron A or B, and for (2) conducting recombination spot tests to determine by deletion mapping the A-segments within which the rll mutations occur. Exercises are recommended for an individual, a team of 2 or 3 students, or use as a demonstration. Other students, however, can participate when they are given sets of result plates for personal analysis and conclusions. Results are obtained from 4 spot tests and 2 control plates. During the course of the experimental procedure described, up to 8 additional sets of 6 plates each can be easily prepared for distribution to other students. Kit provides enough phage and bacteria for such duplication of results, but the required agar plates and soft agar must be purchased separately. Includes a prepaid coupon to request perishable materials at a later date. Note: Please refer to Bacteriophage Media and item #124730 Plaque Assay Plating Set. Refrigerate coliphage until use; do not freeze. Keep bacteria culture and agar at room temperature.

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What's Included

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
1 x 108 T4rllA Known Mutant Tester, 3 mL 1 Included
1 x 109 T4rllAB Reference Deletion Mutant X, 3 mL 1 Included
1 x 109 T4rllAB Reference Deletion Mutant Y, 3 mL 1 Included
1 x 109 T4rllAB Reference Deletion Mutant Z, 3 mL 1 Included
1 x 109 T4rll Unknown Mutant No. 1, 5 mL 1 Included
1 x 109 T4rll Unknown Mutant No. 2, 5 mL 1 Included
1 x 109 T4rll Unknown Mutant No. 3, 5 mL 1 Included
E. coli B, Agar Slant Culture 1 Included
E. coli K12 (lambda), Agar Slant Culture 1 Included
Nutrient Peptone Broth, 80 mL 1 Included
Base Layer Agar, 125 mL 2 Included
Sterile Petri Dishes 20 Included
Dropper Vial, 5 mL 1 Included
Glass Spotting Rods, 150 mm 21 Included
Sterile Disposable Dropping Pipets 3 Included
Screw-cap Vials (labeled for genetic crosses) 6 Included
Transfer Pipets, 1 mL 35 Included
Bulb, 1 cc 3 Included
Tubes Broth, 5 mL 3 Included
Tubes Broth for 10-Fold Dilutions, 9 mL 4 Included
Tubes Broth for 100-Fold Dilutions, 9.9 mL 13 Included
Soft Agar, 2 mL 10 Included
Autoclave Disposable Bag 1 Included
Wax Marker 1 Included
Procedure Flow Sheets 10 Included
Instruction Manuals with Worksheets 10 Included

Resources

How-To & Techniques

Living Organism Care Information

Sample Teacher Manuals

 

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