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Advanced—For students with moderate experience in microbiology.
For a class of 30. Transformation is a form of recombination in which free DNA is released from donor cells, taken up by a recipient's cells, and incorporated into the recipient's genome. Students treat streptomycin-resistant bacterial cells with a detergent to lyse the cells, thus releasing the DNA. Then they incubate streptomycin-sensitive bacterial cells in contact with free DNA. The streptomycin-sensitive cells are transformed and can then grow in the presence of streptomycin as streptomycin-resistant cells. Before rehydration, each MicroKwik® Culture can be kept at room temperature for 2 months or under refrigeration for 6 months.Pathogen (Human or Animal). Cannot be shipped to high schools, preparatory schools, or individuals, in accordance with guidelines of the US Department of Health and Human Services.
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