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BeginningEasy to perform; requires no experience in microbiology.
Antibiotics are chemicals produced by one microbe that inhibit other microbes. Students study the effect of 6 different antibiotics on a Gram-positive bacterium and a Gram-negative bacterium.
Gather laboratory evidence of bacterial antibiotic sensitivity and construct an explanation for how antibiotic resistant bacteria evolve.
PE: HS-LS4-4
Time Requirement: 3 45-minute Class Periods
SEP: Constructing Explanations and Designing Solutions
CCC: Cause and Effect
DCI: LS4.B: Natural Selection
BeginningEasy to perform; requires no experience in microbiology.
Antibiotics are chemicals produced by one microbe that inhibit other microbes. Students study the effect of 6 different antibiotics on a Gram-positive bacterium and a Gram-negative bacterium. Exercise requires 3 consecutive days to complete. Perfect for introducing beginner students to microbiology and antibiotic production. This Demonstration Kit, designed for one teacher demonstration or a single student or group, includes comprehensive teacher's manual with reproducible student guide, as well as FREE 1-year access to digital resources that support 3-dimensional instruction according to the NGSS.
Note: Kit ships with perishable materials included. Keep bacteria cultures at room temperature; do not incubate or refrigerate. Refrigerate antibiotic disks until use; do not freeze.
Components | Qty | Included? |
---|---|---|
Bacillus cereus Broth Culture ![]() |
1 | Included |
Escherichia coli Broth Culture ![]() |
1 | Included |
Ampicillin Antibiotic Disks ![]() |
2 | Included |
Chloramphenicol Antibiotic Disks ![]() |
2 | Included |
Gentamycin Antibiotic Disks ![]() |
2 | Included |
Penicillin Antibiotic Disks ![]() |
2 | Included |
Streptomycin Antibiotic Disks ![]() |
2 | Included |
Tetracycline Antibiotic Disks ![]() |
2 | Included |
Nutrient Agar, 20-mL vials | 2 | Included |
Sterile Petri Dishes | 2 | Included |
White Control Disk | 1 | Included |
Forceps | 1 | Included |
Autoclave Disposal Bag | 1 | Included |
Techniques for Studying Bacteria and Fungi Manual | 1 | Included |
Carolina Science Online® Resources (1-year subscription) | 1 | Included |
Teacher's Manual with Reproducible Student Guide | 1 | Included |
Metric Rulers | 1 | Needed, Not Included |
Labeling Marker | 1 | Needed, Not Included |
Bunsen Burner or Alcohol Burner | 1 | Needed, Not Included |
Water Bath with Thermometer | 1 | Needed, Not Included |
Test Tube Rack | 1 | Needed, Not Included |
Heat-Resistant Gloves, Pair | 1 | Needed, Not Included |
Paper Towels | Needed, Not Included | |
Goggles | Needed, Not Included | |
Lab Apron | Needed, Not Included | |
Gloves | Needed, Not Included | |
Matches | Needed, Not Included | |
Cellophane Tape | Needed, Not Included | |
Disinfectant (70% ethanol or bleach) | Needed, Not Included | |
Incubator (optional) | Needed, Not Included | |
Autoclave (optional) | Needed, Not Included |
Select quality media products for the application you need.