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Safe classroom exercise for students to produce a new viable bacteria strain. Genetic manipulation used is like more complex procedures in modern genetic engineering. Through bacterial conjugation, substituted sex factors are transferred to a background genome expressing antibiotic resistance. Indicator and selective agar media identify and isolate hybrid cells. Cultures need incubation during lab; prior to lab, keep cultures at room temp. With voucher for cultures. For 6 student teams.

A safe classroom exercise in which students produce a new strain of viable bacteria. The genetic manipulation conducted is similar to more complex procedures common in modern genetic engineering. Through bacterial conjugation, substituted sex factors (F plasmids bearing lac operon genes) are transferred to a background genome expressing antibiotic resistance. Indicator and selective agar media are used to identify and isolate the hybrid cells. As part of the lab, cultures will require incubation. Heterogenotic bacteria constructed in this exercise can be tested by the procedure outlined in item #171027 Carolina BioKits™: Introduction to Gene Regulation: The lac Operon. Designed for 6 teams of students. Note: Prior to lab, keep bacteria cultures at room temperature; do not incubate or refrigerate.

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