Advanced—For experienced high school and college classes; requires some technical skill.
This biology lab explores the engineering of a yeast that's genetically modified to produce ß-carotene. Use PCR to test the metabolic pathway of modified Saccharomyces cerevisiae as part of the engineering design-build-test pathway and investigate how to make it possible for users to bake vitamin A-enriched loaves of bread.

Advanced—For experienced high school and college classes; requires some technical skill.

• Introduce bioengineering concepts
• Teach synthetic biology and microbiology techniques
• Allow students to experience real-world connections

This biology lab explores the engineering of a yeast that's genetically modified to produce ß-carotene. In the body, ß-carotene is converted to vitamin A, which is crucial for vision, the immune system, and other biological functions. Use PCR to test the metabolic pathway of modified Saccharomyces cerevisiae as part of the engineering design-build-test pathway and investigate how to make it possible for users to bake vitamin A-enriched loaves of bread. Kit includes enough materials for 24 students working in 6 groups. The BioBuilder® Golden Bread PCR Electrophoresis Pack (item #217009, sold separately) is required to perform the kit's electrophoresis activity. Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact us or return the voucher to request delivery of perishable materials.