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The vision of the Bioscience Core Skills Institute (BCSI) is a future where students, trainees, and employees can accurately and reliably document the skills they acquire. To do this, BCSI provides workforce skills assessment and digital micro-credentialing that is valid, reliable, and trusted by the bioscience industry. The BCSI Credential Evaluation Kit: Aseptic Technique: Lab Bench Level supplies sufficient materials for 1 testing station to service up to 20 tests.

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The vision of the Bioscience Core Skills Institute (BCSI) is a future where students, trainees, and employees can accurately and reliably document the skills they acquire. To do this, BCSI provides workforce skills assessment and digital micro-credentialing that is valid, reliable, and trusted by the bioscience industry. Learn more about BCSI micro-credentialing.

The BCSI Credential Evaluation Kit: Aseptic Technique: Lab Bench Level supplies sufficient materials for 1 testing station to service up to 20 tests.

Aseptic Technique: Lab Bench Level: Transfer of sterile solutions and cultures at the lab bench.

Objectives Measured

• Prepare the lab bench surface for aseptic benchwork.
• Handle instruments such as Bunsen burner (or equivalent), striker, inoculating loop, test tubes, serological pipet, pipet pump, and agar plates appropriately.
• Aseptically transfer a bacterial colony to a culture tube with broth media.
• Aseptically transfer a volume of sterile broth to a new sterile tube.

Performance Assessed

• Student will be given the task of transferring a bacterial colony.
• All materials are located and arranged to be efficient while transferring bacteria.
• Bunsen burner is lit appropriately and adjusted if necessary.

Credential Alignment
Texas Biomanufacturing Skills Standards

• 2.1.2 Personal protection equipment (PPE) such as gloves, eye protection, aprons, and respirators are worn as needed.
• 2.1.4 Appropriate agent(s) and amounts for cleaning are used.
• 4.6.4 Proper aseptic techniques are followed.
• 6.1.2 Microbial growth is identified.
• 6.1.7 Basic microbiology lab techniques are performed.

Washington Skill Standards

• A4. Maintain biological stock cultures.
• A6. Prepare biological and/or chemical materials.
• B5. Handle and/or maintain biological stock cultures.

BACE (Biotechnician Assistant Credentialing Exam)
Skill Category: Biotechnology

• Demonstrate proper aseptic/sterile technique.
• Accurately measure liquids using micropipette and serological pipets.
• Use of inoculating loop.
• Use of agar plate media.
• Use of culture tube with broth media.
• Handling of agar plate lid and test tube lids.
• Appropriate use of an inoculating loop to obtain a bacterial colony.
• Measure the volume indicated on a serological pipet (range of 1 to 9 mL).
• Aseptically transfer the volume to a new sterile tube.