Students amplify their own mitochondrial DNA for sequencing in this kit, which teaches real-world applications of PCR, such as those used in forensics and the analysis of ancient DNA samples. Students examine a sequence within one of the hypervariable regions of the mitochondrial genome (mtDNA). They use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they amplify a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome.

Advanced—For experienced high school and college classes; requires some technical skill.

Updated to include PCR Master Mix in place of PCR beads!

Generate excitement by having your students amplify their own mitochondrial DNA for sequencing! The Mitochondrial DNA Polymorphisms in Human Evolution with Master Mix Kit enlivens discussions on the molecular basis of heredity and evolution by placing both concepts directly into the hands of students as they learn and practice authentic biotechnology techniques. The kit uses a simple and robust protocol that is ideal for the classroom and teaches real-world applications of PCR, such as those used in forensics and the analysis of ancient DNA samples.

This experiment examines a sequence within one of the hypervariable regions of the mitochondrial genome (mtDNA). First, students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they amplify a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome, which contains numerous single nucleotide polymorphisms (SNPs). After gel electrophoresis confirms amplification, the student samples may be sent for sequencing to analyze SNPs that vary from person to person (the cost of sequencing is not included in the price of the kit). Students then compare their SNPs to ancient hominids and to people from different world populations to discover patterns of DNA variation and to analyze theories of human evolution.

Each kit contains sufficient materials for 25 reactions and includes updated DNALC resources, including a paper model activity, a visual lab procedure, a real-world connections section, and support for obtaining BCSI biotechnology microcredentials.

Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact Carolina or return the voucher to request delivery of perishable materials.