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This plasmid contains the gene for ampicillin resistance as well as the entire ß-galactosidase gene. It does not depend on complementing a partial ß-galactosidase gene product as does pUC. In addition, the host, E. coli DH5, does not need to be maintained on minimal medium for pBLU® transformation. The lac suppressor gene is inactivated, eliminating the need for IPTG in the medium.