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Description
This plasmid contains the gene for ampicillin resistance as well as the entire ß-galactosidase gene. It does not depend on complementing a partial ß-galactosidase gene product as does pUC. In addition, the host, E. coli DH5, does not need to be maintained on minimal medium for pBLU® transformation. The lac suppressor gene is inactivated, eliminating the need for IPTG in the medium.
Developed by Dr. Greg Freyer (coauthor of DNA Science: A First Course—2nd Edition, item #212209) exclusively for Carolina. This plasmid contains the gene for ampicillin resistance as well as the entire ß-galactosidase gene. It does not depend on complementing a partial ß-galactosidase gene product as does pUC. In addition, the host, E. coli DH5, does not need to be maintained on minimal medium for pBLU® transformation. The lac suppressor gene is inactivated, eliminating the need for IPTG in the medium. *Transformation concentration; use 10 µL/transformation. pBLU® Plasmid, 10 µg (50 µL; 0.2 µg/µL)