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Protein Assay 8-Station Kit

(in stock)


Intermediate - Easy to perform; requires some background knowledge.

Students use a standard curve to analyze data in this experiment. They gather data by performing a BCA assay to determine the amount of protein in a standard versus 3 different food samples (included). Optionally, they can supply and test a fourth liquid food sample of their choosing. A spectrophotometer is used to obtain numerical values and absorbances of dilutions of the standard and the samples. Students graph absorbance versus protein concentration for the standard and use the resulting curve to interpolate the concentration of protein in the samples. Kit includes enough materials for 8 stations of students.


What’s Included:
  • 1 Reagent A, 305 mL
  • 1 Reagent B, 7 mL
  • 1 BSA Standard, 2 mg/mL, 1.4 mL
  • 1 Powdered Milk
  • 1 Diet Drink
  • 1 High-protein Health-food Drink
  • 48 BSA Dilution Tubes (1.5 mL microcentrifuge tubes)
  • 136 Assay and Food Product Dilution Tubes, 15 mL
  • 8 Pipets, 5 mL
  • 8 Pipets, 10 mL
  • 1 Teacher's Manual and Reproducible Student Guide
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Needed But Not Included:
  • 8 Containers for Holding 37 mL of Reagent A
  • 8 Containers for Holding 0.780 mL of Reagent B
  • 8 Rulers
  • 8 Trays
  •  Spectrophotometer(s) (absorbance at 562 nm)
  •  Pipet Bulb or other Pipetting Device
  •  Pipets to measure 20 to 780 μL
  •  Water Baths, 37 or 60°C
  •  Distilled or Deionized Water
  •  Racks
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