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Examining the RNAi Mechanism Kit (RNAi and Amplification; with Perishables)

$317.30
(in stock)

Description

Advanced- For experienced high school and college classes; requires some basic technical skill.

  • Demonstrate to your students the power of silencing a single gene
  • Teach about a powerful method for determining gene function
  • Explore the relationship between phenotype, genotype, and RNAi-induced phenotype
  • Perform bioinformatics exercises exploring protein function and C. elegans, and human relatedness

RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. Examining the RNAi Mechanism kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the nonparasitic roundworm C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, dumpy 13 (dpy-13).

Kit ships with the perishable materials included. Kit also includes FREE 1-year access to digital resources that support 3-dimensional instruction for NGSS.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This is a special order item that requires additional time for culturing and processing before it ships. Arrival timeframes are available during Checkout.
What’s Included:
  • 1 Ampicillin, 10 mg/mL, 4 mL  
  • 2 Cholesterol, 5 mg/mL, 0.3 mL  
  • 1 IPTG, 0.036 g  
  • 1 Proteinase K, 20 mg/mL, 25 μL  
  • 1 DNA Marker pBR322/BstN1, 200 μL  
  • dpy-13 (e458) Worms on NGM-lite Plates  
  • dpy-13 Primer/Loading Dye Mix, 700 μL  
  • E. coli RNA Feeding Strain (dpy-13) LB Amp/Tet Slant  
  • E. coli OP50 on LB Slant  
  • 4 Wild-type Worms on NGM-lite Plates  
  • 1 1x Lysis Buffer, 0.5 mL
  • 6 Sterile Bulb Pipets
  • 3 Sterile Culture Tubes, 15 mL
  • 3 Sterile Inoculating Loops
  • 3 LB Broth, 3 mL
  • 1 Mineral Oil, 5 mL
  • 1 Melt 'n Pour NGM-lite, 300 mL
  • 1 Melt 'n Pour NGM-lite, 100 mL
  • 40 Sterile Petri Dishes, 60 mm
  • 25 Ready-to-Go™ PCR Beads (0.2 mL tubes)
  • 1 Sterile Distilled Water, 1 mL
  • 8 Sterile Toothpicks
  • 1 Teacher's Manual and Reproducible Student Guide
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Needed But Not Included:
  •  Document Camera or Overhead Projector
  •  Stereomicroscope
  •  Bunsen Burner
  •  Containers with Cracked or Crushed Ice
  •  Container with Liquid Nitrogen or Dry Ice
  •  Digital or Instant Camera (optional)
  •  Ethanol, 95%
  •  Gel Casting Trays and Combs
  •  Gel Electrophoresis Chamber
  •  Masking Tape
  •  Microcentrifuge
  •  Microcentrifuge Adaptors
  •  Micropipettor and Tips, 1-20 μL and 20-200 μL
  •  Microcentrifuge Tube Rack
  •  Mineral Oil
  •  PCR Tubes, 0.2 or 0.5 μL
  •  Permanent Markers, Black and Red
  •  Power Supply
  •  Steel Scalpel, Spatula, or Forceps
  •  Thermal Cycler
  •  UV or White Light Transilluminator
  •  Water Bath, 60°C (for agarose solution)
  •  Heavy-duty Plastic Bag
  •  Household Bleach
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Return Policy:

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