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Advanced—For students with moderate experience in microbiology.

Students treat streptomycin-resistant bacterial cells with a detergent to lyse the cells, thus releasing the DNA. Then they incubate streptomycin-sensitive bacterial cells in contact with free DNA. The streptomycin-sensitive cells are transformed and can then grow in the presence of streptomycin as streptomycin-resistant cells.

Advanced—For students with moderate experience in microbiology.

For a class of 30. Transformation is a form of recombination in which free DNA is released from donor cells, taken up by a recipient's cells, and incorporated into the recipient's genome. Students treat streptomycin-resistant bacterial cells with a detergent to lyse the cells, thus releasing the DNA. Then they incubate streptomycin-sensitive bacterial cells in contact with free DNA. The streptomycin-sensitive cells are transformed and can then grow in the presence of streptomycin as streptomycin-resistant cells. Before rehydration, each MicroKwik® Culture can be kept at room temperature for 2 months or under refrigeration for 6 months.Pathogen (Human or Animal). Cannot be shipped to high schools, preparatory schools, or individuals, in accordance with guidelines of the US Department of Health and Human Services.

   This item is sold and shipped to colleges and universities only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

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