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Students use RNAi to silence the dpy-13 gene in the non-parasitic roundworm, C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, DumPY 13 (dpy-13). The ingestion of the dsRNA from the bacteria initiates the destruction of mRNA expressed from the targeted dpy-13 gene. Comes with perishable materials.

Advanced- For experienced high school and college classes; requires some basic technical skill.

• Demonstrate to your students the power of silencing a single gene
• Teach about a powerful method for determining gene function
• Explore the relationship between phenotype, genotype, and RNAi-induced phenotype
• Perform bioinformatics exercises exploring protein function and C. elegans, and human relatedness

RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. Examining the RNAi Mechanism Kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the nonparasitic roundworm C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, DumPY 13 (dpy-13).

Kit ships with the perishable materials included. Kit also includes FREE 1-year access to digital resources that support 3-dimensional instruction for NGSS.

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