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Description

Use methylation-sensitive enzymes to explore epigenetics—heritable changes in gene expression—that affect flowering in Arabidopsis. Using techniques such as DNA extraction, restriction enzyme digest, PCR, gel electrophoresis, and bioinformatics, students learn the pivotal role DNA methylation plays in gene expression. Each kit contains materials for 25 DNA extractions and PCR reactions and materials for electrophoresis using CarolinaBLU®.

Advanced—For experienced high school and college classes; requires some technical skill.

  • Introduce students to the growing field of epigenetics
  • Gain experience working with the model plant Arabidopsis thaliana
  • Investigate the role of DNA methylation in gene regulation
  • Use a protocol designed especially for the classroom
Utilize methylation-sensitive enzymes to explore epigenetics—heritable changes in gene expression—that affect flowering in Arabidopsis. By growing wild-type Ler and mutant fwa-1 plants, students are able to observe phenotypic differences in flowering and then investigate this difference using multiple molecular techniques. Students conduct DNA extraction, restriction enzyme digest, PCR, gel electrophoresis, and bioinformatics to investigate the pivotal role DNA methylation plays in gene regulation. Kit contains materials for 25 DNA extractions and PCR materials for electrophoresis using CarolinaBLU®. Note: Kit includes a voucher to request perishables later at your convenience. Contact us or return the voucher to request delivery of perishable materials. Kit must be used within 4 weeks of receiving it.

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