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IntermediateEasy to perform; requires some background knowledge.
Students separate mixtures of precut DNA fragments using samples of lambda DNA - uncut, cut with EcoRI, and cut with HindIII - to effectively demonstrate the action of restriction enzymes and the principles of electrophoresis. Provides sufficient materials for 2 demonstrations.
IntermediateEasy to perform; requires some background knowledge.
Exploring electrophoresis of DNA demonstration kit. Students separate mixtures of precut DNA fragments using samples of lambda DNA - uncut, cut with EcoRI, and cut with HindIII - to effectively demonstrate the action of restriction enzymes and the principles of electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with 1 battery.
This Demonstration Kit contains instructions, 1 set of apparatus (with 4-well comb), and sufficient materials for 2 demonstrations.