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Description
Students set up their own restriction digests of lambda DNA with BamHI, EcoRI, and HindIII using Instant DNA and Instant Enzymes. They then separate the resulting DNA fragments by electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with one battery.
Intermediate - Easy to perform; requires some background knowledge.
Students set up their own restriction digests of lambda DNA with BamHI, EcoRI, and HindIII using Instant DNA and Instant Enzymes. They then separate the resulting DNA fragments by electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with one battery. The Demonstration Kit contains instructions, one set of apparatus, and sufficient supplies for 2 separate experiments. The Classroom Kit contains instructions, 5 sets of apparatus (with 4-well comb), and sufficient supplies for 10 separate experiments. The Replacement Kit contains enough supplies for 10 experiments.
